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PE anti-mouse CD30 Antibody

Pricing & Availability
Clone
mCD30.1 (See other available formats)
Regulatory Status
RUO
Other Names
Ber-H2, Ki-1, TNFRSF8
Isotype
Armenian Hamster IgG
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Product Citations
publications
mCD30_1_PE_081908.jpg
ConA-stimulated C57BL/6 splenocytes (Day 3) stained with mCD30.1 PE
  • mCD30_1_PE_081908.jpg
    ConA-stimulated C57BL/6 splenocytes (Day 3) stained with mCD30.1 PE
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102306 200 µg 297 CHF
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Description

CD30 is a 105 kD type I transmembrane glycoprotein, also known as Ber-H2 or Ki-1. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on activated T, B, and NK cells, and monocytes. CD28, in conjunction with several cytokines, regulates the expression of CD30 on activated T cells. It has been reported that cytokine production and Th1 versus Th2 development is heavily influenced by CD30-CD30 ligand (CD153) interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD30-mouse IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 102306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 102304) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Bowen MA, et al. 1996. J. Immunol. 156:442.
  2. Punt JA, et al. 1997. J. Exp. Med. 186:1911.
Product Citations
  1. Lyu J, et al. 2023. Mol Biol Cell. br11:34. PubMed
RRID
AB_2207612 (BioLegend Cat. No. 102306)

Antigen Details

Structure
TNFR superfamily, 105-120 kD
Distribution

Activated T cells, B cells and NK cells, monocytes

Function
Costimulation, regulates negative selection of thymocytes
Ligand/Receptor
CD153 (CD30L)
Cell Type
B cells, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Falini B, et al. 1995. Blood 85:1.
3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.

Gene ID
21941 View all products for this Gene ID
UniProt
View information about CD30 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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