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PE anti-mouse CD366 (Tim-3) Recombinant Antibody

Pricing & Availability
Clone
QA20A20 (See other available formats)
Regulatory Status
RUO
Other Names
CD366, Tim-3, Hepatitis A virus cellular receptor 2 homolog (HAVcr-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_QA20A20_PE_CD366_Antibody_1_111523
Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3) recombinant (clone QA20A20) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • A_QA20A20_PE_CD366_Antibody_1_111523
    Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3) recombinant (clone QA20A20) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • B_QA20A20_PE_CD366_Antibody_2_111523
    BALB/c splenocytes were cultured for 4 days in the presence of plate-bound purified anti-mouse CD3e, soluble purified anti-mouse CD28, and purified anti-mouse IL-4. Then cells were stained with anti-mouse CD8a APC and anti-mouse CD366 (Tim-3) (clone QA20A20) PE (right) or mouse IgG1, κ PE isotype control (left).
See PE spectral data
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165403 25 µg 110 CHF
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165404 100 µg 255 CHF
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA20A20 completely blocks the binding of reference clone RMT3-23 on target cells.

RRID
AB_3083286 (BioLegend Cat. No. 165403)
AB_3083286 (BioLegend Cat. No. 165404)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain with a predicted molecular weight of 31 kD; can exist as a soluble form lacking mucin and transmembrane domains.
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Interaction
Macrophages, Th1
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Sabatos CA, et al. 2003. Nat Immunol. 4:1102-10.
  2. Kuchroo VK, et al. 2003. Nat Rev Immunol. 3:454-62.
  3. Mooney L, et al. 2002. Nature. 415:536-41.
Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.15.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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