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PE anti-mouse CD9 Antibody

Pricing & Availability
Clone
MZ3 (See other available formats)
Regulatory Status
RUO
Other Names
DRAP-27, MRP-1, p-24
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MZ3_PE_CD9_Antibody_1_102324
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cyanine5.5 and MZ3 PE
  • MZ3_PE_CD9_Antibody_1_102324
    C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cyanine5.5 and MZ3 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
124805 50 µg 98 CHF
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124806 200 µg 317 CHF
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Description

CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other membrane proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Won WJ, et al. 2002. J. Immunol. 168:5605. (FC, WB)
Product Citations
  1. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  2. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  3. Jiang L, et al. 2016. Nat Commun. 7:13045. PubMed
  4. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  5. Etzerodt A, et al. 2017. Sci Rep. 7:40286. PubMed
  6. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  7. Visan KS, et al. 2022. J Extracell Vesicles. 11:e12266. PubMed
  8. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
  9. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  10. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  11. Molgora M, et al. 2020. Cell. 182:886. PubMed
  12. Kosicki M, et al. 2018. Nat Biotechnol. 36:765. PubMed
  13. Chavez JS, et al. 2022. Cells. 11:. PubMed
  14. Pritykin Y, et al. 2021. Molecular Cell. 81(11):2477-2493.e10. PubMed
  15. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  16. Zaslavsky A, et al. 2013. Blood. 121:3205. PubMed
RRID
AB_1279327 (BioLegend Cat. No. 124805)
AB_1279327 (BioLegend Cat. No. 124806)

Antigen Details

Structure
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution

CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.

Interaction
CD9 may participate in forming large molecular complexes with other associated molecules.
Cell Type
B cells, Embryonic Stem Cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, Platelets, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Boucheix C, et al. 1991. J. Biol. Chem. 266:117
2. Lanza F, et al. 1991. J. Biol. Chem. 266:10638

Gene ID
12527 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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