PE anti-mouse H-2Kd/H-2Dd Antibody anti-H-2Ld, H-2Db

Pricing & Availability

Clone: 34-1-2S (See other available formats)
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
Ave. Rating: Submit a Review
Product Citations: publications

34-1-2s — C57BL/6 mouse splenocytes stained with 34-1-2S PE

Compare all formats See PE spectral data

Cat #

Size

Price

Quantity Check Availability

Save

114708

200 µg

329 CHF

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

The 34-1-2S antibody reacts with the H-2K^d/H-2D^d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K^d/H-2D^d haplotype. H-2K^d/H-2D^d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation², complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
RRID: AB_313607 (BioLegend Cat. No. 114708)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^d/H-2D^d haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821.

Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2Kd/H-2Dd on UniProt.org

Documentation

Reviews

Review This Product

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All H-2Kd/H-2Dd Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Alexa Fluor® 647 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PerCP/Cyanine5.5 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
PE/Cyanine7 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
APC/Fire™ 750 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC
Spark Violet™ 423 anti-mouse H-2K^d/H-2D^d	34-1-2S	FC

Certificate of Analysis

Customers Also Purchased

PE anti-mouse H-2K^b/H-2D^b

Clone: 28-8-6

PE/Dazzle™ 594 anti-mouse CD163 Antibody

Clone: S15049I

PE/Fire™ 640 anti-mouse CD4

Clone: GK1.5

Alexa Fluor® 700 anti-mouse CD206 (MMR)

Clone: C068C2

Go To Top Version: 2 Revision Date: 04.10.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.