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PE anti-mouse/human Helios Antibody

Pricing & Availability
Clone
22F6 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype
Armenian Hamster IgG
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Product Citations
publications
22F6_PE_031210
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), and then intracellularly stained with Helios-PE (clone 22F6).
  • 22F6_PE_031210
    C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), and then intracellularly stained with Helios-PE (clone 22F6).
  • 22F6_PE_060710
    Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), and then intracellulary stained with Helios-PE (clone 22F6).
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137206 25 tests 214 CHF
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137216 100 tests 420 CHF
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Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Helios peptide (aa 51-107)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. It is recommended that the reagent be titrated for optimal performance for each application.
For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
  2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
  3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
  4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
  5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
  6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
  7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations
  1. Hatam L, et al. 2012. Clin Cancer Res. 2.086805556. PubMed
  2. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  3. Takimoto Y, et al. 2023. iScience. 26:106220. PubMed
  4. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  5. McClymont S, et al. 2011. J Immunol. 186:3918. PubMed
  6. Zeng Z, et al. 2016. J Exp Med. 213: 1079 - 1093. PubMed
  7. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  8. Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed
  9. MaruYama T 2015. Biochem Biophys Res Commun. 464: 586-589. PubMed
  10. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  11. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  12. Baine I, et al. 2013. J Immunol. 190:1008. PubMed
  13. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  14. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  15. Sawanobori Y, et al. 2021. Histochem Cell Biol. 156:133. PubMed
  16. Zeng Z, et al. 2013. J Immunol. 191:4184. PubMed
  17. Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed
  18. Dehzad N, et al. 2011. J Immunol. 186:2238. PubMed
  19. Wraith J 2010. J Immunol. 185:7129. PubMed
  20. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  21. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  22. Paiva R, et al. 2013. Proc Natl Acad Sci U S A. 110:6494. PubMed
  23. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  24. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  25. Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed
  26. Ostadkarampour M, et al. 2016. PLoS One. 11:e0164751. PubMed
  27. Redpath S, et al. 2015. Infect Immun . 83: 3881 - 3889. PubMed
  28. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  29. Kim YC, et al. 2018. J Immunol. 201:1671. PubMed
  30. Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed
  31. Adel–Patient K, et al. 2018. Clin Transl Allergy. 8:38. PubMed
  32. Zhao J, et al. 2011. J Exp Med. 208:1571. PubMed
  33. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
RRID
AB_10552903 (BioLegend Cat. No. 137206)
AB_10552903 (BioLegend Cat. No. 137216)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al.  1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02.03.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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