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PE anti-mouse/human IL-5 Antibody

Pricing & Availability
Clone
TRFK5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
Isotype
Rat IgG1, κ
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Product Citations
publications
TRFK5_PE_IL-5_Antibody_101322
Th2 polarized BALB/c splenocytes were stimulated with PMA + ionomycin for 6 hours and then were stained with anti-mouse CD3 APC, and intracellularly stained with anti-mouse IL-5 (clone TRFK5) PE (left) or rat IgG1 PE isotype control (right).
  • TRFK5_PE_IL-5_Antibody_101322
    Th2 polarized BALB/c splenocytes were stimulated with PMA + ionomycin for 6 hours and then were stained with anti-mouse CD3 APC, and intracellularly stained with anti-mouse IL-5 (clone TRFK5) PE (left) or rat IgG1 PE isotype control (right).
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504303 25 µg 96 CHF
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504304 100 µg 283 CHF
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Guinea Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partially-purified T cell clone supernatant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1,3,4 or ELISPOT2,6 Capture: The purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated TRFK4 antibody (Cat. No. 504402) as the detection antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody (Cat. No. 501002) as the detection antibody for human IL-5. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled TRFK51 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.

Neutralization1,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity (Cat. Nos. 504313-504318).

Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections8 and paraformaldehyde-fixed, saponin-treated frozen tissue sections7, and immunocytochemistry.

Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 and 430404) are specially developed and recommended. For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA MAX™ Sets (Cat. Nos. 431201 and 431206) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  2. Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
  3. Swain, S., et al. 1990. J. Immunol. 145:3796.
  4. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  5. Assenmacher, M., et al. 1994. Eur. J. Immunol. 24:1097.
  6. Karulin, A., et al. 2000. J. Immunol. 164:1862.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Fan, W. Y., et al. 2001. Exp. Biol. Med. 226:1045.
  9. Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Product Citations
  1. Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed
  2. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  3. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  4. Amelio P, et al. 2017. PLoS Negl Trop Dis. 10.1371/journal.pntd.0005817. PubMed
  5. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  6. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  7. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
  8. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  9. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  10. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  11. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  12. Shami A, et al. 2015. Sci Rep. 5: 13904. PubMed
  13. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  14. Dai G, et al. 2021. mSphere. 6: . PubMed
  15. Li A, et al. 2020. J Virol. 94:00:00. PubMed
  16. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  17. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  18. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  19. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  20. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  21. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  22. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  23. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  24. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  25. Garcia-Dominguez D, et al. 2022. Front Immunol. 13:948335. PubMed
  26. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  27. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
RRID
AB_315328 (BioLegend Cat. No. 504303)
AB_315328 (BioLegend Cat. No. 504304)

Antigen Details

Structure
Cytokine; dimer; 40-50 kD (Mammalian)
Bioactivity
Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, eosinophils
Cell Targets
Eosinophils, B cells
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.

Gene ID
16191 View all products for this Gene ID 3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All IL-5 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human IL-5 TRFK5 ICFC
PE anti-mouse/human IL-5 TRFK5 ICFC
Purified anti-mouse/human IL-5 TRFK5 ELISA Capture,CyTOF®,IHC,WB
Purified anti-mouse/human IL-5 (Maxpar® Ready) TRFK5 ELISA Capture,CyTOF®
Brilliant Violet 421™ anti-mouse/human IL-5 TRFK5 ICFC
Ultra-LEAF™ Purified anti-mouse/human IL-5 TRFK5 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC,IHC,WB
Go To Top Version: 2    Revision Date: 10.13.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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