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PE anti-mouse/human KLRG1 (MAFA) Antibody

Pricing & Availability
Clone
2F1/KLRG1 (See other available formats)
Regulatory Status
RUO
Other Names
MAFA, 2F1-Ag
Isotype
Syrian Hamster IgG
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Product Citations
publications
2F1_KLRG1_PE_1_051710
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and 2F1/KLRG1 PE
  • 2F1_KLRG1_PE_1_051710
    C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and 2F1/KLRG1 PE
  • 2F1_KLRG1_PE_2_051710
    C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and Syrian hamster IgG PE isotype control
  • 2F1_KLRG1_PE_KLRG1_Antibody_FC_1_010813
    Human peripheral blood lymphocytes were stained with CD56 APC and KLRG1 (clone 2F1/KLRG1) PE.
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138407 25 µg 104 CHF
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138408 100 µg 304 CHF
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Description

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag).  KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3.  It is composed of a homodimer of glycosylated 30-38 kD subunits.  Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells.  KLRG1 may be involved in regulating NK cell homeostasis.  KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
IL-2 activated NK cells from C57BL/6 mice
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.

Additional reported applications (for relevant formats) include: Western Blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
  2. Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
  3. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed
  3. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  4. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  5. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  6. Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
  7. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  8. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  9. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  10. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  11. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  12. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
  13. Parriott G, et al. 2020. Immunology. 160:280. PubMed
  14. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  15. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  16. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  17. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  18. Zhang Y, et al. 2014. Infect Immun . 82:3033. PubMed
  19. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  20. Zhao H, et al. 2021. Int J Nanomedicine. 16:4913. PubMed
  21. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  22. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  23. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  24. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  25. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  26. Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
  27. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  28. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  29. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  30. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
RRID
AB_10574313 (BioLegend Cat. No. 138407)
AB_10574313 (BioLegend Cat. No. 138408)
Disclaimer

This product may be used for research purposes only.  It is not licensed for resale and may only be used by the buyer.  This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service.  If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California.  The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution

Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.

Function
Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand/Receptor
E-cadherin
Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Antigen References

1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834.

Gene ID
50928 View all products for this Gene ID
UniProt
View information about KLRG1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 02.11.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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