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PE anti-mouse PLZF Antibody

Pricing & Availability
Clone
9E12 (See other available formats)
Regulatory Status
RUO
Other Names
Promyelocytic leukemia zinc finger, Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
9E12_PE_PLZF_Antibody_FC_1_032613
C57BL/6 mouse leukocytes from mouse liver were surface stained with CD45 PerCP, CD8 Alexa Fluor® 488, and NK1.1 Brilliant Violet 421™. Cells were then fixed and permeabilized with 1% PFA plus 70% ethanol, followed by intracellular staining with PLZF (clone 9E12) PE or Armenian hamster IgG PE isotype control. Data shown was gated on CD45+/CD8+ positive cells.
  • 9E12_PE_PLZF_Antibody_FC_1_032613
    C57BL/6 mouse leukocytes from mouse liver were surface stained with CD45 PerCP, CD8 Alexa Fluor® 488, and NK1.1 Brilliant Violet 421™. Cells were then fixed and permeabilized with 1% PFA plus 70% ethanol, followed by intracellular staining with PLZF (clone 9E12) PE or Armenian hamster IgG PE isotype control. Data shown was gated on CD45+/CD8+ positive cells.
  • 9E12_PE_PLZF_Antibody_FC_2_032613
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Cat # Size Price Quantity Check Availability Save
145803 25 µg 153 CHF
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145804 100 µg 360 CHF
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Description

PLZF (promyelocytic leukemia zinc finger), also known as Zbtb16, Zinc finger and BTB domain-containing protein 16, Zfp145, is a member of the BTB-POZ family of transcription factors. It was first identified in a patient with acute promyelocytic leukemia, where a reciprocal chromosomal translocation t(11;17)(q23;q21) resulted in a fusion with RARA gene encoding retinoic acid receptor alpha. Expression of this transcriptional repressor in immune cells differs between human and mouse. In humans, PLZF is expressed in CD34+ progenitor cells and in primitive multipotent hematopoietic cell lines, NK cells, γ/δ T cells, CD4+ and CD8+ T cells. It is also expressed in MR1-specific mucosal-associated invariant T cells as well as in MHC class II-restricted T cells that develop via a thymocyte-thymocyte interaction. PLZF is involved in NK cell function, cellular quiescence, and growth suppression. It also inhibits gene expression induced by retinoic acid receptor. In mice, PLZF is highly expressed in immature CD1d-resricted invariant NKT (iNKT) cells, a subset of γ/δ (Vg1.1+Vd6.3+) T cells, and non-invariant CD1d-restricted T cells. PLZF exists as a homodimer or in complex with PLZP, and has been shown to be involved in the development of NKT cells. It is also reported to be expressed in embryonic tissues, giving rise to hematopoietic progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Combination of peptides covering the amino, carboxyl and hinge regions of the PLZF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis using 1% PFA plus 70% ethanol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  2. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  3. Anderson CK et al. 2019. Cell Rep. 27(2):537-548 . PubMed
  4. Park JY, et al. 2019. Cell Rep. 27:2548. PubMed
  5. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
RRID
AB_2561966 (BioLegend Cat. No. 145803)
AB_2561966 (BioLegend Cat. No. 145804)

Antigen Details

Structure
BTB domain-containing
Distribution
iNKT cells, γ/δ (Vg1.1+Vd6.3+) T cells, non-invariant CD1d-restricted T cells
Function
Involved in NKT cell development
Cell Type
NKT cells, T cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Constantinides MG, et al. 2011. J. Immunol. 187:309.
2. Kovalovsky D, et al. 2008. Nat. Immunol. 9:1055.
3. Chen Z, et al. 1993. EMBO J. 12:1161.
4. Chen Z, et al. 1994. Proc. Natl. Acad. Sci. USA 91:1178.

Gene ID
235320 View all products for this Gene ID
UniProt
View information about PLZF on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 04.20.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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