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PE anti-mouse Podocalyxin Antibody

Pricing & Availability
Clone
10B9 (See other available formats)
Regulatory Status
RUO
Other Names
Podocalyxin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
10B9_PE_Podocalyxin_Antibody_021822
bEND.3 cells were stained with anti-mouse Podocalyxin (clone 10B9) PE (filled histogram) or rat IgG1, κ isotype control PE (open histogram).
  • 10B9_PE_Podocalyxin_Antibody_021822
    bEND.3 cells were stained with anti-mouse Podocalyxin (clone 10B9) PE (filled histogram) or rat IgG1, κ isotype control PE (open histogram).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
107903 25 µg 193 CHF
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107904 100 µg 288 CHF
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Description

Podocalyxin is sialomucin, most closely related to CD34 and endoglycan, expressed by kidney podocytes, hematopoietic progenitors, vascular endothelia, and a subset of neurons. It regulates both cell adhesion and cell morphology and plays an essential role in the formation of podocyte foot processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
OSM-dependent endothelial-like cell line, LO
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Onitsuka, I., et al., 2010. Gastroenterology, 138:1525-1535.
  2. Doyonnas, R., et al., 2005. Blood, 105:4170-4178.
  3. Minegishi, N., et al., 2003. Blood, 102:896-905.
  4. Minehata, K., et al., 2002. Blood, 99:2360-2368.
  5. Doyonnas, R., et al., 2001. J. Exp. Med., 194:13-28.
  6. Hara, T., et al., 1999. Immunity, 11:567-578.
RRID
AB_2922457 (BioLegend Cat. No. 107903)
AB_2922457 (BioLegend Cat. No. 107904)

Antigen Details

Structure
Single-pass type I membrane protein, sialoglycoprotein
Distribution

Apical cell membrane, membrane raft

Function
Cell adhesion, cell migration
Interaction
Forms a complex with EZR and SLC9A3R2
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References
  1. Refaeli I, et al. 2020. Scientific Reports 10:9419
  2. Economou C, et al. 2004. J Cell Sci (2004) 117 (15): 3281–94.
  3. Cait J, et al. 2019. Proc Natl Acad Sci. 116 (10) 4518-4527
  4. Doyonnas R, et al. 2005. Blood. 105(11):4170-8
  5. Zhang H, et al. 2014. Stem Cells.  32(1):191-203
Gene ID
27205 View all products for this Gene ID
UniProt
View information about Podocalyxin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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