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PE anti-mouse/rat XCR1 Antibody

Pricing & Availability
Clone
ZET (See other available formats)
Regulatory Status
RUO
Other Names
GPR5, CCXCR1, mXCR1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
ZET_PE_XCR1_Antibody_FC_1_061614
Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c Brilliant Violet 421™, CD8α APC and XCR1 (clone ZET) PE (top) or mouse IgG2b, κ PE isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_PE_XCR1_Antibody_FC_1_061614
    Cells from collagenase-digested C57BL/6 mouse spleen were stained with CD11c Brilliant Violet 421™, CD8α APC and XCR1 (clone ZET) PE (top) or mouse IgG2b, κ PE isotype control (bottom). Data shown was gated on the CD11c+ population.
  • ZET_PE_XCR1_Antibody_FC_2_061614
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148203 25 µg 153 CHF
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148204 100 µg 335 CHF
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Description

XCR1, also known as GPR5 or CCXCR1, is a 38 kD, G-protein coupled, seven transmembrane receptor, and the only member of the "C" chemokine receptor family. XCR1 mediates chemotaxis of XCL1 and XCL2 (lymphotactin-1 and -2), and defines a subset of CD8α+ conventional dendritic cells capable of antigen cross-presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissues2.

Application References

(PubMed link indicates BioLegend citation)
  1. Gurka S, et al. 2015. Front Immunol. 6:35. (FC)
  2. Kitazawa Y, et al. 2019. Front Immunol. 10:1195. (FC, IHC-F)
Product Citations
  1. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  2. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  3. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  4. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  5. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  6. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  7. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  8. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  9. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  10. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  11. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  12. Sharma MD, et al. 2018. Immunity. 48:91. PubMed
  13. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  14. Lin H, et al. 2021. Cancer Cell. 39:480. PubMed
  15. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  16. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  17. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  18. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  19. Huang X, et al. 2021. Immunity. . PubMed
  20. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
RRID
AB_2563842 (BioLegend Cat. No. 148203)
AB_2563842 (BioLegend Cat. No. 148204)

Antigen Details

Structure
Only member of the "C" chemokine receptor family, seven transmembrane receptor, coupled to G protein, 38 kD.
Distribution

Subset of CD8α+ conventional dendritic cells.

Function
Mediates chemotaxis to XCL1, defines a population of CD8α+ dendritic cells capable of antigen cross-presentation to CD8+ T cells.
Ligand/Receptor
XCL1 and XCL2.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yamazaki C, et al. 2013. J. Immunol. 190:6071.
2. Shimizu K, et al. 2013. J. Immunol. 190:5609.
3. Bachem A, et al. 2012. Front. Immunol. 3:214.
4. Crozat K, et al. 2011. J. Immunol. 187:4411.

Gene ID
23832 View all products for this Gene ID 301086 View all products for this Gene ID
UniProt
View information about XCR1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08.13.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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