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PE anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
h57-597
C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC
  • h57-597
    C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ (UC7-13D5) FITC
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109207 50 µg 73 CHF
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109208 200 µg 219 CHF
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Collin R, et al. 2020. J Immunol. 205:133. PubMed
  3. Poh AR, et al. 2022. Sci Adv. 8:eabl7882. PubMed
  4. Campisi L, et al. 2022. Nature. 606:945. PubMed
  5. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  6. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  7. Zindl CL, et al. 2022. Immunity. 55:494. PubMed
  8. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  9. Stone VM, et al. 2022. iScience. 25:105070. PubMed
  10. Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed
  11. Axelrod ML, et al. 2022. Nature. 611:818. PubMed
  12. Tokatlian T, et al. 2022. Cancer Res Commun. 2:58. PubMed
  13. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
  14. Levi N, et al. 2023. Aging (Albany NY). 15:2395. PubMed
  15. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
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  17. Thilakasiri P, et al. 2019. EMBO Mol Med. 11:4. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Wolf B, et al. 2015. J Immunol. 195: 2540-2551. PubMed
  20. Choi J, et al. 2021. Cell Rep Methods. 1:100084. PubMed
  21. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  22. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  23. Wagner KI, et al. 2022. Cell Rep. 38:110214. PubMed
  24. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  25. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  26. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  27. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  28. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  29. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  30. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  31. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  32. Tanno H, et al. 2020. Sci Adv. 6:eaay9093. PubMed
  33. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  34. Tung J, et al. 2013. Nutrition. 20:9007. PubMed
  35. Beil-Wagner J, et al. 2016. Sci Rep. 6:21377. PubMed
  36. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  37. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  38. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  39. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  40. Ding R, et al. 2021. Theranostics. 4957:11. PubMed
  41. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  42. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  43. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  44. Liu J, et al. 2021. Immun Inflamm Dis. 9:299. PubMed
  45. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  46. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
  47. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  48. Manry D, et al. 2022. Front Immunol. 13:826747. PubMed
  49. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  50. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  51. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  52. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  53. Mahlios J, Zhuang Y 2011. Mol Immunol. 49:227. PubMed
  54. Molgora M, et al. 2020. Cell. 182:886. PubMed
  55. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  56. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  57. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  58. Gawish R, et al. 2022. Elife. 11:. PubMed
  59. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  60. Young A, et al. 2017. Cancer Res. 77:4684. PubMed
RRID
AB_313431 (BioLegend Cat. No. 109207)
AB_313431 (BioLegend Cat. No. 109208)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All TCR β chain Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TCR β chain H57-597 FC
Biotin anti-mouse TCR β chain H57-597 FC,IHC-F
FITC anti-mouse TCR β chain H57-597 FC,IHC-F
PE anti-mouse TCR β chain H57-597 FC
PE/Cyanine5 anti-mouse TCR β chain H57-597 FC
Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
Alexa Fluor® 488 anti-mouse TCR β chain H57-597 FC,IHC-F
Alexa Fluor® 647 anti-mouse TCR β chain H57-597 FC,IHC-F
APC/Cyanine7 anti-mouse TCR β chain H57-597 FC
PE/Cyanine7 anti-mouse TCR β chain H57-597 FC
Alexa Fluor® 700 anti-mouse TCR β chain H57-597 FC
Pacific Blue™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 421™ anti-mouse TCR β chain H57-597 FC,IHC-F
PerCP/Cyanine5.5 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 570™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 510™ anti-mouse TCR β chain H57-597 FC,IHC-F
Purified anti-mouse TCR β chain (Maxpar® Ready) H57-597 FC,CyTOF®
Alexa Fluor® 594 anti-mouse TCR β chain H57-597 FC,IHC-F
PE/Dazzle™ 594 anti-mouse TCR β chain H57-597 FC
Brilliant Violet 605™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 711™ anti-mouse TCR β chain H57-597 FC
APC/Fire™ 750 anti-mouse TCR β chain H57-597 FC
TotalSeq™-A0120 anti-mouse TCR β chain H57-597 PG
Brilliant Violet 785™ anti-mouse TCR β chain H57-597 FC
Brilliant Violet 650™ anti-mouse TCR β chain H57-597 FC
Ultra-LEAF™ Purified anti-mouse TCR β chain H57-597 FC,CyTOF®,IHC-F,IP,Costim,Depletion
TotalSeq™-C0120 anti-mouse TCR β chain H57-597 PG
TotalSeq™-B0120 anti-mouse TCR β chain H57-597 PG
Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark PLUS UV395™ anti-mouse TCR β chain H57-597 FC
TotalSeq™-D0120 anti-mouse TCR β chain H57-597 PG
Spark Blue™ 574 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Spark Blue™ 550 anti-mouse TCR β chain (Flexi-Fluor™) H57-597 FC
Go To Top Version: 2    Revision Date: 02.06.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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