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PE anti-mouse TCR Vα3.2 [b, c] Antibody

Pricing & Availability
Clone
RR3-16 (See other available formats)
Regulatory Status
RUO
Other Names
Vκ 3.2[b,c] TCR [va3.2]
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_RR3-16_PE_110609
C57BL/6 mouse lymph node cells stained with RR3-16 PE and CD3 (145-2C11) APC
  • A_RR3-16_PE_110609
    C57BL/6 mouse lymph node cells stained with RR3-16 PE and CD3 (145-2C11) APC
  • B_RR3-16_PE_110609
    C57BL/6 lymph node cells stained with rat IgG2b PE isotype control and CD3 (145-2C11) APC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
135405 50 µg 129 CHF
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135406 200 µg 317 CHF
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Description

TCR is a heterodimer composed of alpha and beta chains or gamma and delta chains. TCR Vα elements expression is largely independent of MHC haplotype. It is likely that the Vαs interact preferentially with conserved region of class I or class II molecules. RR3-16 antibody reacts with the Vα3.2 T-cell receptor (TCR) of mice having the b (e.g., C57BL/6) and c (e.g.,SJL, NOD) haplotypes, but not with the strains having the a ( Balb/c) or d (DBA/2) haplotype. Vα3.2 TCR is preferentially expressed by CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse cytolytic T-cell clone OH6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Utsunomiya Y, et al. 1991. Immunogenetics. 33(3):198
  2. Utsunomiya Y, et al. 1989. J. Immunol. 143:2602
Product Citations
  1. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  2. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
  3. Miyauchi E, et al. 2020. Nature. 585:102. PubMed
RRID
AB_1937256 (BioLegend Cat. No. 135405)
AB_1937256 (BioLegend Cat. No. 135406)

Antigen Details

Distribution

subset of T cells.

Function
In combination of V β, Vκ3.2 recognizes MHC/peptide complex on the antigen-presenting cells.
Ligand/Receptor
TCR recognizes MHC/peptide complex.
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Utsunomiya Y, et al. 1989. J. Immunol. 143:2602
2. Sim BC, et al. 1997. J. Immunol. 159(7):3322
3. Iwamoto A, et al. 1987. J. Exp. Med. 165(3):591

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR Valpha3.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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