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- Clone
- B21.14 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor Vα 8.3
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
BALB/c mouse splenocytes stained with CD3 (145-2C11) APC and B21.14 PE
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 127708 | 100 µg | 196 CHF | ||||
The B21.14 antibody recognizes an epitope of the CDR1 on the Va 8.3 subfamily of TCR having the a,b,c and d haplotypes of Tcra gene complex.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Soluble TCR from mouse CTL clone CW3/1.1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Necker A, et al. 1991. Eur J. Immunol. 21:3035
- Brodnicki TC, et al. 1996. Mol Immunol 33:253
- Tomonari K, et al. 1992. Immunogenetics 35:291
- Product Citations
-
- RRID
-
AB_1186072 (BioLegend Cat. No. 127708)
Antigen Details
- Structure
- TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
- Distribution
-
On a subset of T cells
- Function
- Recognize MHC/peptide complex on the antigen-presenting cells.
- Interaction
- Associate with CD3 complex to form TCR complexes expressing on cell surface.
- Ligand/Receptor
- TCR recognizes MHC/peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Brodnicki TC, et al. 1996. Mol Immunol 33:253 - Gene ID
- 110469 View all products for this Gene ID
- UniProt
- View information about TCR Valpha8.3 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All TCR Vα8.3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| FITC anti-mouse TCR Vα8.3 | B21.14 | FC |
| PE anti-mouse TCR Vα8.3 | B21.14 | FC |
| TotalSeq™-C0982 anti-mouse TCR Vα8.3 | B21.14 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
FITC anti-mouse TCR Vα8.3
C57BL/6 splenocytes stained with CD3 (145-2C11) PE and B21.1... -
PE anti-mouse TCR Vα8.3
BALB/c mouse splenocytes stained with CD3 (145-2C11) APC and... -
TotalSeq™-C0982 anti-mouse TCR Vα8.3
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