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PE anti-mouse TCR Vβ6 Antibody

Pricing & Availability
Clone
RR4-7 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor beta 6
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
RR4-7_PE_060710
C57BL/6 splenocytes stained with RR4-7 PE and CD3 APC
  • RR4-7_PE_060710
    C57BL/6 splenocytes stained with RR4-7 PE and CD3 APC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
140003 25 µg 129 CHF
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140004 100 µg 232 CHF
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Description

Vβ6 T cell receptor (TCR Vβ6) is a variant of TCR β chain and, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of the T cell-mediated immune responses. Murine Vβ6 cells recognize the Mtv-7, Mls-1, and rabies superantigens.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
H-Y specific TH Clone OI11
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: induction of T cell proliferation1, blocking in vitro cytolysis1, and in vivo blocking of syngeneic T cell tumor growth2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanagawa O and Palmer EBJ. 1989. Cell. Immunol. 119:412. (Activ, Block)
  2. Kanagawa O. 1989. J. Exp. Med. 170:1513. (Block)
Product Citations
  1. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  2. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  3. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  4. Denzin LK et al. 2017. Immunity. 47(2):310-322 . PubMed
  5. Wang X, et al. 2022. Elife. 11:. PubMed
RRID
AB_10640727 (BioLegend Cat. No. 140003)
AB_10640727 (BioLegend Cat. No. 140004)

Antigen Details

Structure
Member of the immunoglobulin superfamily, Vβ6 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
Distribution

Expressed on a subset of TCR αβ+ T cells.

Function
Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune responses.
Ligand/Receptor
MHC-Peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Acha-Orbea H and MacDonald HR. 1995. Annu. Rev. Immunol. 13:459.
2. Rudy CK, et al. 1992. J. Exp. Med. 175:1613.
3. Jones LA, et al. 1990. Science 250:1726.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR Vbeta6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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