PE anti-Phosphotyrosine Antibody anti-Phosphotyrosine

Pricing & Availability

Clone: PY20 (See other available formats)
Regulatory Status: RUO
Other Names: p-Tyr
Isotype: Mouse IgG2b, κ
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Product Citations: publications

PY20_PE_Phosphotyrosine_Antibody_032116 — Human T leukemia cell line Jurkat was cultured in serum-free media overnight and treated with 5mM H₂O₂ (filled histogram) or PBS (open histogram) for 15 minutes. The cells were then fixed with 2% paraformaldehyde, permeabilized with 90% methanol and stained with anti-phosphotyrosine (clone PY20) PE.

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309310

100 µg

275 CHF

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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat, All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated phosphotyrosine
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, immunocytochemistry³.
RRID: AB_2572196 (BioLegend Cat. No. 309310)

Antigen Details

Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID: 5800 View all products for this Gene ID
UniProt: View information about Phosphotyrosine on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All Phosphotyrosine Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-Phosphotyrosine	PY20	WB
Purified anti-Phosphotyrosine	PY20	WB,ICC,ICFC,IP
Alexa Fluor® 488 anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 647 anti-Phosphotyrosine	PY20	ICFC
APC anti-Phosphotyrosine	PY20	ICFC
PE anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 594 anti-Phosphotyrosine	PY20	ICC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

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