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PE anti-rat CD47 Antibody

Pricing & Availability
Clone
OX-101 (See other available formats)
Regulatory Status
RUO
Other Names
gp42, IAP, neurophilin
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
OX-101_PE_110308.jpg
LOU rat splenocytes stained with OX-101 PE
  • OX-101_PE_110308.jpg
    LOU rat splenocytes stained with OX-101 PE
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204108 100 µg 238 CHF
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Description

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment, and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. The significance of this molecule is recently drawing increasing attention. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. DeVries HE, et al. 2002. J. Immunol. 168:5832.
RRID
AB_2074948 (BioLegend Cat. No. 204108)

Antigen Details

Structure
50 kD plasma membrane protein of immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids.
Distribution

Broad tissue distribution, including haemopoietic cells, epithelial and endothelial cells, fibroblasts, erythrocytes and other tissue cells.

Function
Adhesion molecules, immune regulation
Interaction
Associate with β3 integrins
Ligand/Receptor
SIRPα (CD172α, signal regulatory protein alpha), thrombospondin
Cell Type
Epithelial cells, Endothelial cells, Fibroblasts, Erythrocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Vernon-Wilson, et al. 2000. Eur. J. Immunol. 30:2130.
  2. Brown E, et al. 1990. J. Cell. Biol. 111:2785.
  3. Mawby WJ, et al. 1994. Biochem. J. 304:525.
Gene ID
16423 View all products for this Gene ID
UniProt
View information about CD47 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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