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PE anti-RPS6 Antibody

Pricing & Availability
Clone
A16009C (See other available formats)
Regulatory Status
RUO
Other Names
40S ribosomal protein S6, Ribosomal Protein S6
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16009C_PE_RPS6_Antibody_051818
HEK 293T cells were fixed, permeabilized, and intracellularly stained with anti-RPS6 (clone A16009C) PE (filled histogram) or purified Mouse IgG2b, κ PE isotype control (open histogram).
  • A16009C_PE_RPS6_Antibody_051818
    HEK 293T cells were fixed, permeabilized, and intracellularly stained with anti-RPS6 (clone A16009C) PE (filled histogram) or purified Mouse IgG2b, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
691803 25 tests 141 CHF
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691804 100 tests 372 CHF
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Description

The eukaryotic ribosome is composed of 40S and 60S subunits, which is involved in the physiological process of protein translation. In many organisms, ribosomal proteins undergo various post-translational modifications, including phosphorylation, acetylation, methylation, O-linked b-N-acetylglucosaminylation, and ubiquitylation. Ribosomal proteinS6 (RPS6), the first identified ribosomal protein which can be phosphorylated, is a component of eukaryotic ribosomal 40S subunit. Functional studies of RPS6 phosphorylation suggested its role in the regulation of protein synthesis, cell proliferation, and glucose homeostasis. The phosphorylation of RPS6 can be triggered by multiple stimuli including growth factors, tumor promoting agents, and mitogens. The inhibition of RPS6 phosphorylation was found mainly under stress conditions such as hypoxia, heat shock, and hyperosmolarity. RPS6 phosphorylation is also commonly used as a marker for neuronal activity and a read out of mammalian target of rapamycin complex 1 (mTORC1) activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human S6 peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2734535 (BioLegend Cat. No. 691803)
AB_2734535 (BioLegend Cat. No. 691804)

Antigen Details

Structure
249 amino acids with a predicted molecular weight of 28.7kD.
Distribution

Cytoplasm and nucleus.

Function
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Kressler A, et al. 2010. Biochim. Biophys. Acta. 1803:673.
2. Biever A, et al. 2015. Front. Mol. Neurosci. 8:75.
3. Ventimiglia FA, Wool IG. 1974. Proc. Natl. Acad. Sci. USA 71:350.
4. Lastick SM, et al. 1977. Mol. Gen. Genet. 152:223.
5. Ruvinsky I, et al. 2005. Genes Dev. 19:2199.
6. Meyuhas O. 2008. Int. Rev. Cell. Mol. Biol. 268:1.
7. Meyuhas O. 2015. Int. Rev. Cell. Mol. Biol. 320:41.

Gene ID
6194 View all products for this Gene ID
UniProt
View information about RPS6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All RPS6 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-RPS6 A16009C WB,ICFC,KO/KD-WB,IHC-P
PE anti-RPS6 A16009C ICFC
Go To Top Version: 1    Revision Date: 05.21.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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