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PE/Cyanine7 anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
4B4-1 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-7
Other Names
4-1BB, ILA, CD137, TNFRSF9
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
4B4-1_PECy7_040810
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with 4B4-1 PE/Cyanine7
  • 4B4-1_PECy7_040810
    PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with 4B4-1 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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309817 25 tests 153 CHF
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309818 100 tests 323 CHF
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ectodomain of recombinant human 4-1BB fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
  2. Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
  3. Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
  4. Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
Product Citations
  1. Chacon J, et al. 2015. Clin Cancer Res. 21:611. PubMed
  2. Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed
  3. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  4. Nogimori T, et al. 2023. Front Immunol. 13:1081047. PubMed
  5. Schnizer C 2022. Frontiers in Immunology. 13:1095129. PubMed
  6. Qiu C, et al. 2022. Front Bioeng Biotechnol. 10:1027619. PubMed
  7. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  8. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  9. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  10. Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
  11. Tischer S, et al. 2012. Int Immunol. 24:561. PubMed
  12. Afanasiev O, et al. 2013. Clin Cancer Res. 19:5351. PubMed
  13. Sandt C, et al. 2016. J Virol. 90: 1009 - 1022. PubMed
  14. Hodge G, et al. 2013. J Heart Lung Transplant. 1053:1357. PubMed
  15. Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed
  16. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  17. Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed
  18. Menges D, et al. 2022. Nat Commun. 13:4855. PubMed
  19. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  20. Lecciso M, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01918. PubMed
RRID
AB_2207741 (BioLegend Cat. No. 309817)
AB_2207741 (BioLegend Cat. No. 309818)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand
Cell Type
T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839.

Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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