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PE/Cyanine7 anti-human CD154

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone
24-31
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype
Mouse IgG1, κ
24-31_PE_Cyanine7_CD154_ASR_090524
Typical results from PMA + Ionomycin without Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes stained either with 24-31 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
  • 24-31_PE_Cyanine7_CD154_ASR_090524
    Typical results from PMA + Ionomycin without Brefeldin A stimulated (4-hour) human peripheral blood lymphocytes stained either with 24-31 PE/Cyanine7 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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984404 500 µL 467 CHF
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Description

CD154 is the ligand for CD40 (CD40L), also know as gp39, TRAP and T-BAM. It is a 32-39 kD type II transmembrane glycoprotein, belonging to the TNF superfamily. CD154 is expressed on activated T cells. It is important for B cell co-stimulation following binding to its receptor, CD40. Additionally, binding of CD154 to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD154 is also involved in the regulation of cytokine production by T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Formulation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), True-Stain Monocyte Blocker™, and a stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
100 µg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

Suggested for Flow Cytometry
Disclaimer

WARNINGS AND PRECAUTIONS

  1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
  2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
  3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
  4. Do not use this reagent beyond the expiration date stated on the label.
  5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
  6. Avoid prolonged exposure of the reagent or stained cells to light.

Antigen Details

Antigen References
  1. Gauchat JF, et al. 1993. FEBS Lett. 315:259-66.
  2. Hollenbaugh D, et al. 1992. EMBO J. 11:4313-21.
  3. Parker DC. 1993. Annu Rev Immunol. 11:331-60.
Go To Top Version: 1    Revision Date: 10.11.2024

Analyte Specific Reagent. Analytical and performance characteristics are not established.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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