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PE/Cyanine7 anti-human CD163 Antibody

Pricing & Availability
Clone
RM3/1 (See other available formats)
Regulatory Status
RUO
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/haptoglobin complex receptor, Macrophage-associated antigen, ED2(rat), Macrophage marker
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RM3slash1_PECy7_CD163_Antibody_FC_122214
Human peripheral blood monocytes were incubated overnight with IL-10 and then stained with anti-human CD163 (clone RM3/1) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • RM3slash1_PECy7_CD163_Antibody_FC_122214
    Human peripheral blood monocytes were incubated overnight with IL-10 and then stained with anti-human CD163 (clone RM3/1) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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326513 25 tests 147 CHF
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326514 100 tests 347 CHF
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kuffer cells, monocytes, subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammation diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human monocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RM3/1 binds to domain 9 of CD163. Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Högger P, et al. 1998. J. Immunol. 161:1883. (FC)
  2. Zwadlo G, et al. 1987. Exp. Cell Biol. 55:295. (FC)
  3. Buechler C, et al. 2000. J. Leukoc. Biol. 67:97. (FC)
  4. Puig-Kroger A, et al. 2009. Cancer Res. 69:9395. (FC) PubMed
  5. Madsen M, et al. 2004. J. Biol. Chem. 279:51561. (FC)
  6. Jones K, et al. 2013. Clin Cancer Res. 19:731. (FC) PubMed
  7. Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
Product Citations
  1. Wang X, et al. 2022. Oncogene. 41:4259. PubMed
  2. Li J, et al. 2023. Adv Sci (Weinh). e2202956:10. PubMed
  3. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  4. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
RRID
AB_2565244 (BioLegend Cat. No. 326513)
AB_2565244 (BioLegend Cat. No. 326514)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, Scavenger receptor superfamily
Distribution
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Monocytes, Macrophages, Dendritic cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994. Transolantation. 57:127.
2. Van den Heuvel MM, et al.1999. J. Leukoc. Biol. 66:858.
3. Sulahian TH, et al. 2000. Cytokines 12:1312.
4. Fabriek BO, et al. 2007. J. Neuroimmunol. 187:179.

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org
Go To Top Version: 1    Revision Date: 01.02.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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