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PE/Cyanine7 anti-human CD194 (CCR4) Antibody

Pricing & Availability
Clone
L291H4 (See other available formats)
Regulatory Status
RUO
Other Names
CKR4, K5-5, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
L291H4_PECyanine7_CD194_Antibody_1_041024
Human peripheral blood lymphocytes were stained with CD3 FITC and CD194 (clone L291H4) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • L291H4_PECyanine7_CD194_Antibody_1_041024
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD194 (clone L291H4) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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359409 25 tests 147 CHF
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359410 100 tests 347 CHF
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Description

CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4- Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4+ T cells leads to Th1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CCR4 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  2. Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
  3. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  4. Vitanza NA, et al. 2021. Nature Medicine. :. PubMed
  5. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  6. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  7. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  8. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  9. Schultheiß C, et al. 2020. Immunity. 53(2):442-455.e4.. PubMed
  10. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
RRID
AB_2562430 (BioLegend Cat. No. 359409)
AB_2562430 (BioLegend Cat. No. 359410)

Antigen Details

Structure
GPCR, seven transmembrane receptor
Distribution

Expressed on a subset of T and B cells, basophils, monocytes, NK cells

Function
Migration of inflammatory and regulatory cells to the target tissues
Ligand/Receptor
CCL17 and CCL22
Cell Type
B cells, Basophils, Embryonic Stem Cells, Monocytes, NK cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Katschke KJ, et al. 2001 Arthritis Rheum. 44:1022.
2. Colantonio L, et al. 2002 Eur. J. Immunol. 32:1264.
3. Jakubzick C et al. 2004 Am. J. Pathol. 165:1211.
4. Morimoto Y, et al. 2005 J. Leukoc. Biol. 78:753.
5. Baatar J, et al. 2007 J. Immunol. 178:4891.
6. Kusumoto M, et al. 2007 J. Interferon. Cytokine Res. 27:901.

Gene ID
1233 View all products for this Gene ID
UniProt
View information about CD194 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 10.10.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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