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PE/Cyanine7 anti-human CD277 (BTN3A) Recombinant Antibody

Pricing & Availability
Clone
34-7.Rec (See other available formats)
Regulatory Status
RUO
Other Names
BTF5, BT3.1, BTN3.1, BT3.2, BTF4, BTN3.2, BTF3, BTN3.3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
34-7Rec_PE-CYA7_CD277_FC_122923
Human peripheral blood lymphocytes were stained with anti-human CD277 (BTN3A) recombinant (clone 34-7.Rec) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • 34-7Rec_PE-CYA7_CD277_FC_122923
    Human peripheral blood lymphocytes were stained with anti-human CD277 (BTN3A) recombinant (clone 34-7.Rec) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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383205 25 tests 206 CHF
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383206 100 tests 429 CHF
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Description

Butyrophilin subfamily 3A (BTN3A), also known as CD277, is a 56 kD type 1 transmembrane glycoprotein. The butyrophilin (BT)/B7 3A family includes three members, BTN3A1, BTN3A2, and BTN3A3, which share a highly conserved extracellular IgV-like domain. BTN3A is broadly expressed in various immune cells including T cells, B cells, NK cells, monocytes, dendritic cells, endothelial cells, and some tumor cells. It plays a role in T cell activation, regulating their proliferation and release of IFNƳ and other cytokines.

Clone 34-7.Rec has been shown to bind to a V region of BTN3A3 that is shared with BTN3A1 and BTN3A2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
pRC-CMV-BTN3A3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This product was formerly named “anti-human CD277 (BTN3A1).” The 34-7.Rec clone has been confirmed to recognize BTN3A3. Cross-reactivity with other BTN3A subfamily members has not been tested.

RRID
AB_3097466 (BioLegend Cat. No. 383205)
AB_3097466 (BioLegend Cat. No. 383206)

Antigen Details

Structure
56 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
Distribution

Cell membrane

Function
Signaling receptor binding, Homodimerization
Interaction
TFF1, PEPL, BT3A2, G5E9A7
Biology Area
Immuno-Oncology, Inhibitory Molecules
Molecular Family
CD Molecules, Tumor Suppressors
Antigen References
  1. Vantourout P, et al. 2018. Proc Natl Acad Sci USA. 115:1039-44.
  2. Payne KK, et al. 2020. Science. 369: 942-9.
  3. Rigau M, et lat. 2020. Science. 367:eaay5516.
  4. Yang Y, et al. 2019. Immunity. 50:1043-53.
Gene ID
11119 View all products for this Gene ID 10384 View all products for this Gene ID
UniProt
View information about CD277 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.16.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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