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PE/Cyanine7 anti-human Granzyme A Antibody

Pricing & Availability
Clone
CB9 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CB9_PECyanine7_Granzyme-A_Antibody_1_111523
Human peripheral blood lymphocytes were surface stained with CD8 Brilliant Violet 421™, fixed and permeabilized (Fixation Buffer Cat. No. 420801, Permeabilization Wash Buffer Cat. No. 421002) and then stained with Granzyme A (clone CB9) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • CB9_PECyanine7_Granzyme-A_Antibody_1_111523
    Human peripheral blood lymphocytes were surface stained with CD8 Brilliant Violet 421™, fixed and permeabilized (Fixation Buffer Cat. No. 420801, Permeabilization Wash Buffer Cat. No. 421002) and then stained with Granzyme A (clone CB9) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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507221 25 tests 165 CHF
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507222 100 tests 375 CHF
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Description

Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting. The CB9 monoclonal antibody recognizes human Granzyme A and has been shown to be useful for flow cytometry, immunoprecipitation, and immunohistochemistry (paraffin-embedded sections).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human Granzyme A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.

Application References

(PubMed link indicates BioLegend citation)
  1. Trimble L, et al. 1998. Blood 91:585.
  2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
  3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
  4. Chen H, et al. 2005. J. Immunol. 175:591.
Product Citations
  1. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  2. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  3. Singh KS, et al. 2021. Nature. 589:597. PubMed
RRID
AB_2721667 (BioLegend Cat. No. 507221)
AB_2721667 (BioLegend Cat. No. 507222)

Antigen Details

Structure
Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
Bioactivity
Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
Cell Sources
Cytotoxic T cells, NK cells
Cell Targets
Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
Receptors
Assisted by perforin
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Enzymes and Regulators, Proteases
Antigen References

1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.

Gene ID
3001 View all products for this Gene ID
UniProt
View information about Granzyme A on UniProt.org
Go To Top Version: 1    Revision Date: 10.20.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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