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PE/Cyanine7 anti-human IL-22 Antibody

Pricing & Availability
Clone
2G12A41 (See other available formats)
Regulatory Status
RUO
Other Names
IL22, IL-D110, IL-TIF, ILTIF, MGC79382, MGC79384, TIFIL-23, TIFa, zcyto18
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2G12A41_PECyanine7_IL-22_Antibody_1_102526
PMA + ionomycin stimulated (6 hours, in the presence of monensin) PBMCs were stained with CD4 APC, fixed, permeabilized, and intracellularly stained with IL-22 (clone 2G12A41) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
  • 2G12A41_PECyanine7_IL-22_Antibody_1_102526
    PMA + ionomycin stimulated (6 hours, in the presence of monensin) PBMCs were stained with CD4 APC, fixed, permeabilized, and intracellularly stained with IL-22 (clone 2G12A41) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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366707 25 tests 159 CHF
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366708 100 tests 405 CHF
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Description

IL-22 is a cytokine that is structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF, also known as the IL-10-related T cell-derived inducible factor. IL-22 belongs to a family of cytokines with limited homology specifically to IL-10, IL-19, IL-20, IL-24, IL-26, the IFN-λs, IL-28A, IL-28B, and IL-29. Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Its activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II cytokine receptor family. Two types of IL-22 binding receptors have been discovered: a membrane-bound receptor and a soluble receptor that are encoded by different genes. IL-22 is produced by Th17 cells and Th22 cells. The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant protein expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  2. Perveen K, et al. 2021. Int J Mol Sci. 0.916666666666667. PubMed
  3. Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed
RRID
AB_2571931 (BioLegend Cat. No. 366707)
AB_2571931 (BioLegend Cat. No. 366708)

Antigen Details

Structure
Cytokine.
Distribution

Thymic lymphomas, T cells, activated T cells, macrophages, mast cells, and Th17.

Function
Enhances the production of acute phase proteins in HepG2 cells and promotes Th17 cells to trasmigrate through the blood-brain barrier in multiple sclerosis.
Interaction
IL-22 receptor.
Ligand/Receptor
IL-22R1 and IL-10R2 receptor chains.
Cell Type
Embryonic Stem Cells, Macrophages, Mast cells, T cells, Th17
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagalakshmi ML, et al. 2004. Int. Immunopharmacol. 5:679.
2. Kebir H,, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene Y, et al. 2008. J. Immunol. 180:7423.
5. Dumotier L, et al. 2000. J. Immune 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 40:31335.
7. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
8. Chung Y, et al. 2006. Cell Res. 11:902.
9. Trifari S, et al. 2009. Nat. Immunol. 10:864.

Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org
Go To Top Version: 1    Revision Date: 03.07.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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