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PE/Cyanine7 anti-mouse CD215 (IL-15Rα) Antibody

Pricing & Availability
Clone
6B4C88 (See other available formats)
Regulatory Status
RUO
Other Names
IL-15RA, IL-15Rα, IL-15 Receptor α subunity
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
6B4C88_PECyanine7_CD215_Antibody_032522
Mouse bone marrow derived dendritic cells were stimulated overnight with LPS. Cells were stained with anti-mouse CD215 (IL-15Rα) (clone 6B4C88) PE/Cyanine7 (filled histogram) or with rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
  • 6B4C88_PECyanine7_CD215_Antibody_032522
    Mouse bone marrow derived dendritic cells were stimulated overnight with LPS. Cells were stained with anti-mouse CD215 (IL-15Rα) (clone 6B4C88) PE/Cyanine7 (filled histogram) or with rat IgG2a, κ PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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153509 25 µg 176 CHF
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153510 100 µg 382 CHF
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Description

IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD215 recombinant protein (33-205 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2922478 (BioLegend Cat. No. 153509)
AB_2922478 (BioLegend Cat. No. 153510)

Antigen Details

Structure
IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology of IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains.
Distribution

It is found on a variety of subsets including dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells.

Function
IL-15Rα is involved in T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
Ligand/Receptor
IL-15
Cell Type
Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Giri JG, et al. 1995. EMBO J. 14:3654.
2. Giron-Michel J, et al. 2005. Blood. 106:2302.
3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642.
4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588.
5. Anderson BG, Quinn LS. 2016. Endocrinology. 157:1315

Gene ID
16169 View all products for this Gene ID
UniProt
View information about CD215 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03.25.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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