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PE/Cyanine7 anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
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Product Citations
publications
10Fdot9G2_PECy7_071410
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 (purple line).
  • 10Fdot9G2_PECy7_071410
    C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE/Cyanine7 (orange line) or rat IgG2b, κ PE/Cyanine7 (purple line).
Compare all formats See PE/Cyanine7 spectral data
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124313 25 µg 99 CHF
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124314 100 µg 292 CHF
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  2. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  3. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  4. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  5. Zhao T, et al. 2023. J Immunother Cancer. 11:. PubMed
  6. Taha Z, et al. 2023. Front Immunol. 14:1181014. PubMed
  7. Liu X, et al. 2020. Nature. . PubMed
  8. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  9. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  10. Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed
  11. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  12. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  13. Xu Y, et al. 2019. Nat Med. 25:301. PubMed
  14. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  15. Zhang D, et al. 2021. Cancer Med. 10:2137. PubMed
  16. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  17. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  18. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  19. Li J, et al. 2018. Immunity. 49:178. PubMed
  20. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  21. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  22. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  23. Zhao T, et al. 2022. JCI Insight. 7:. PubMed
  24. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  25. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  26. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  27. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  28. Fang W, et al. 2021. J Exp Clin Cancer Res. 40:4. PubMed
  29. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  30. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  31. Kahn JH, et al. 2020. Endocrinology. 161(10):. PubMed
  32. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  33. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  34. Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed
RRID
AB_10639934 (BioLegend Cat. No. 124313)
AB_10639934 (BioLegend Cat. No. 124314)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 2    Revision Date: 12.22.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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