PE/Cyanine7 anti-mouse DR3 TNFRSF25 Antibody anti-DR3

Pricing & Availability

Clone: 4C12 (See other available formats)
Regulatory Status: RUO
Other Names: Apo-3, LARD, TNFR25, TRAMP, WSL-1, DR3
Isotype: Armenian Hamster IgG
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Product Citations: publications

4C12_PECyanine7_DR3_Antibody_1_102523 — Mouse splenocytes were stained with CD4 FITC and anti-mouse DR3 (TNFRSF25, clone 4C12) PE/Cyanine7 (left) or Armenian Hamster IgG, PE/Cyanine7 isotype control (right).

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144409

25 µg

171 CHF

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144410

100 µg

370 CHF

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Description

DR3, also known as TNFRSF25, lymphocyte-associated receptor of death (LARD), WSL-1, TRAMP, TR3 and APO3, is a 55 kD glycoprotein belonging to the TNF receptor superfamily (TNFRSF). TNFSF25 is expressed at low level on CD4-positive, CD8-positive T cells and NKT cells. It has been shown to stimulate NF-κB activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domains containing adaptor proteins. Recently, it has been shown that TNFRSF25 is also expressed on Treg cells and regulates Treg proliferation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mtnfr25-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: 4C12 is an agonistic antibody. It leads to rapid and selective proliferation of pre-existing Tregs in vivo through TCR engagement with MHC class II (MHC-II) and IL-2 signaling. We recommend our Ultra-LEAF™ purified 4C12 antibody (Cat. No. 144411 and 144412) for agonist or in vivo studies.
RRID: AB_2734205 (BioLegend Cat. No. 144409)
AB_2734205 (BioLegend Cat. No. 144410)

Antigen Details

Structure: 55 kD glycoprotein, contains one death domain and four TNFR-Cys repeats
Distribution: CD4, CD8, NKT and Tregs at low level
Function: Plays a role in lymphocyte homeostasis, induces NFKB activation and apoptosis
Ligand/Receptor: TL1-A
Cell Type: NKT cells, T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Taraban VY, et al. 2011. J. Clin. Invest. 121:463.

Slebioda TJ, et al. 2011. Eur. J. Immunol. 41:2606.

Schreiber TH, et al. 2010. J. Clin. Invest. 120:3629.

Reddy PB, et al. 2012. J. Virol. 2012. [Epub].
Gene ID: 85030 View all products for this Gene ID
UniProt: View information about DR3 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All DR3 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse DR3 (TNFRSF25)	4C12	FC
APC anti-mouse DR3 (TNFRSF25)	4C12	FC
PE/Cyanine7 anti-mouse DR3 (TNFRSF25)	4C12	FC
Ultra-LEAF™ Purified anti-mouse DR3 (TNFRSF25)	4C12	FC,Agonist
TotalSeq™-C0836 anti-mouse DR3 (TNFRSF25)	4C12	PG
TotalSeq™-B0836 anti-mouse DR3 (TNFRSF25) Antibody	4C12	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05.22.2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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