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PE/Cyanine7 anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RMM-1_PECyanine7_IgM_Antibody_1_041024
C57BL/6 splenocytes stained with CD19 (6D5) APC and IgM (clone RMM-1) PE/Cyanine7 (left) or rat IgG2a isotype control (right).
  • RMM-1_PECyanine7_IgM_Antibody_1_041024
    C57BL/6 splenocytes stained with CD19 (6D5) APC and IgM (clone RMM-1) PE/Cyanine7 (left) or rat IgG2a isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
406513 25 µg 89 CHF
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406514 100 µg 229 CHF
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Thomsen I, et al. 2021. J Immunol. Online ahead of print. PubMed
  2. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  3. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  4. Erickson JJ, et al. 2022. Nature. 606:769. PubMed
  5. Blanco LP, et al. 2022. Arthritis Rheumatol. 74:1971. PubMed
  6. Urata S, et al. 2023. Microbiol Immunol. 67:105. PubMed
  7. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  8. Hong H, et al. 2022. STAR Protoc. 3:101526. PubMed
  9. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  10. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  11. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  12. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  13. Hung PJ, et al. 2018. Mol Cell. 71:332. PubMed
  14. Cunha P, et al. 2022. Front Vet Sci. 9:891893. PubMed
  15. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  16. Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371/journal.pone.0185509. PubMed
  17. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  18. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  19. Li H, et al. 2020. Nature. 584:274. PubMed
  20. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  21. Souza SP, et al. 2021. PLoS Pathog. 17:e1010081. PubMed
  22. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  23. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  24. Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
  25. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  26. Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
  27. Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed
  28. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
RRID
AB_10642031 (BioLegend Cat. No. 406513)
AB_10642031 (BioLegend Cat. No. 406514)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org
Go To Top Version: 2    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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