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PE/Cyanine7 anti-mouse Ly49H Antibody

Pricing & Availability
Clone
3D10 (See other available formats)
Regulatory Status
RUO
Other Names
Klra8, Cmv1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3D10_PECyanine7_Ly49H_Antibody_1_102523
C57BL/6 mouse splenocytes were first treated with True-Stain Monocyte Blocker™ then stained with CD49b (clone DX5) APC and Ly49H (clone 3D10) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cyanine7 Isotype Control (right).
  • 3D10_PECyanine7_Ly49H_Antibody_1_102523
    C57BL/6 mouse splenocytes were first treated with True-Stain Monocyte Blocker™ then stained with CD49b (clone DX5) APC and Ly49H (clone 3D10) PE/Cyanine7 (left) or Mouse IgG1, κ PE/Cyanine7 Isotype Control (right).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
144713 25 µg 89 CHF
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144714 100 µg 235 CHF
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Description

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein.  It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice).  Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice.  Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection.  Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C1498-Ly49A/H transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipation1, and in vitro2 and in vivo3 blocking activity.  The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).

Application References

(PubMed link indicates BioLegend citation)
  1. Guseva NV, et al. 2010. Eur. J. Immunol. 40:2618. (IP)
  2. Orr MT, et al. 2009. J. Exp. Med. 206:807. (Block)
  3. Rodriguez M, et al. 2004. J. Immunol. 173:6312. (Block)
Product Citations
  1. Vetters J, et al. 2023. iScience. 108570:26. PubMed
  2. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  3. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
RRID
AB_2783112 (BioLegend Cat. No. 144713)
AB_2783112 (BioLegend Cat. No. 144714)

Antigen Details

Structure
28.3 kD
Distribution

NK cells but not NKT cells

Function
Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor
m157
Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.

Gene ID
16639 View all products for this Gene ID
UniProt
View information about Ly49H on UniProt.org
Go To Top Version: 1    Revision Date: 10.02.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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