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PE/Cyanine7 anti-mouse P2X7R Antibody

Pricing & Availability
Clone
1F11 (See other available formats)
Regulatory Status
RUO
Other Names
P2X7 receptor (P2X7R), P2RX7
Isotype
Rat IgG2b, κ
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Product Citations
publications
1F11_PECyanine7_P2X7R_Antibody_1_102623
C57BL/6 splenocytes were stained with CD4 APC and P2X7R (clone 1F11) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
  • 1F11_PECyanine7_P2X7R_Antibody_1_102623
    C57BL/6 splenocytes were stained with CD4 APC and P2X7R (clone 1F11) PE/Cyanine7 (left) or rat IgG2b, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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148707 25 µg 159 CHF
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148708 100 µg 370 CHF
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Description

P2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine colon mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1, in vivo inhibition of intestinal inflammation and mast cell activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)
Product Citations
  1. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  2. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  3. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  4. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID
AB_2721685 (BioLegend Cat. No. 148707)
AB_2721685 (BioLegend Cat. No. 148708)

Antigen Details

Structure
Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.
Distribution

Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.

Function
Ion channel activated by extracellular ATP.
Ligand/Receptor
ATP, NAD.
Cell Type
B cells, Macrophages, Mast cells, T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Surprenant A, et al. 1996. Science. 272:735.
2. Chused TM, et al. 1996. J. Immunol. 157:1371.
3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911.
4. Kawamura H, et al. 2006. J. Immunol. 176:2152.
5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908.
6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638.
7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.

Gene ID
18439 View all products for this Gene ID
UniProt
View information about P2X7R on UniProt.org
Go To Top Version: 1    Revision Date: 10.13.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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