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PE/Dazzle™ 594 anti-human CD7 Antibody

Pricing & Availability
Clone
A20153E (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A20153E_PEDazzle594_CD7_Antibody_021424
Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD7 (clone A20153E) PE/Dazzle™ 594 (left) or mouse IgG2b, κ PE/Dazzle™ 594 isotype control (right).
  • A20153E_PEDazzle594_CD7_Antibody_021424
    Human peripheral blood lymphocytes were stained with anti-human CD3 (clone UCHT1) FITC and anti-human CD7 (clone A20153E) PE/Dazzle™ 594 (left) or mouse IgG2b, κ PE/Dazzle™ 594 isotype control (right).
Compare all formats See PE/Dazzle™ 594 spectral data
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385805 25 tests 160 CHF
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385806 100 tests 365 CHF
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Description

CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD7 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone A20153E can completely block binding of clone 4H9-CD7 and partially blocks binding of clone CD7-6B7 on target cells.

RRID
AB_3106032 (BioLegend Cat. No. 385805)
AB_3106032 (BioLegend Cat. No. 385806)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 40 kD
Distribution

T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets

Function
T cell Activation
Cell Type
Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
  2. Stillwell R, et al. 2001. Immunol Res. 24:31-52.
  3. Rabinowich H, et al. 1994. J Immunol. 152:517-26.
Gene ID
924 View all products for this Gene ID
UniProt
View information about CD7 on UniProt.org
Go To Top Version: 1    Revision Date: 02.14.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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