PE/Dazzle™ 594 anti-mouse CD32 (Fcgr2) Antibody, CD32, S17012B

Pricing & Availability

Clone: S17012B (See other available formats)
Regulatory Status: RUO
Other Names: Fcr-2, Fcr-3, Ly-17, LyM-1, Lym-1, fcRII, FcgRII, fcgr2b
Isotype: Rat IgG2b, κ
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Product Citations: publications

S17012B_PEDazzle594_CD32_Antibody_030821 — C57BL/6 mouse splenocytes were stained with anti-mouse CD32 (clone S17012B) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, ᴋ PE/Dazzle™ 594 isotype control (open histogram).

Compare all formats See PE/Dazzle™ 594 spectral data

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Size

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156411

25 µg

141 CHF

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156412

100 µg

347 CHF

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Description

CD32 (Fcgr2) is a 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily. The extracellular region of CD32 consists of two Ig C-type domains that binds the Fc region from monomeric IgG with low affinity, but binds immune complexes efficiently. CD32 can mediate phagocytosis of immune complexes and modulate cell activation. CD32 is expressed by Macrophages, neutrophils, mast cells and B cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD32 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2890717 (BioLegend Cat. No. 156411)
AB_2890717 (BioLegend Cat. No. 156412)

Antigen Details

Structure: 40 kD transmembrane glycoprotein, member of the immunoglobulin superfamily; extracellular region consists of two Ig C-type domains.
Distribution: Macrophages, neutrophils, mast cells and B cells.
Function: Mediates phagocytosis of immune complexes, modulates cell activation.
Ligand/Receptor: Binds Fc from monomeric IgG with low affinity, binds immune complexes efficiently.
Cell Type: B cells, Macrophages, Mast cells, Neutrophils
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Negishi-Koga T, et al. 2015. Nat Commun. 6:6637

Yamada DH, et al. 2015. Immunity. 42:379

Clatworthy MR, et al. 2014. Nat Med. 20:1458

Li F and Ravetch JV. 2011. Science. 333:1030

Xiang Z, et al. 2007. Nat Immunol. 8:419
Gene ID: 14130 View all products for this Gene ID
UniProt: View information about CD32 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD32 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD32 (Fcgr2)	S17012B	FC
PE anti-mouse CD32 (Fcgr2)	S17012B	FC
APC anti-mouse CD32 (Fcgr2)	S17012B	FC
FITC anti-mouse CD32 (Fcgr2)	S17012B	FC
PE/Cyanine7 anti-mouse CD32 (Fcgr2) Antibody	S17012B	FC
PE/Dazzle™ 594 anti-mouse CD32 (Fcgr2) Antibody	S17012B	FC
Alexa Fluor® 647 anti-mouse CD32 (Fcgr2)	S17012B	FC
Alexa Fluor® 700 anti-mouse CD32 (Fcgr2)	S17012B	FC
PerCP/Cyanine5.5 anti-mouse CD32 (Fcgr2)	S17012B	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03.08.2021

For Research Use Only. Not for diagnostic or therapeutic use.

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