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PE/Fire™ 640 anti-mouse CD278 (ICOS) Antibody

Pricing & Availability
Clone
15F9 (See other available formats)
Regulatory Status
RUO
Other Names
Inducible COStimulatory molecule, H4, CD278
Isotype
Syrian Hamster IgG
Ave. Rating
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Product Citations
publications
15F9_PEFire640_CD278_Antibody_050622
C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for three days. Cells were stained with anti-mouse CD278 (ICOS) (clone 15F9) PE/Fire™ 640 (filled histogram) or left unstained (open histogram).
  • 15F9_PEFire640_CD278_Antibody_050622
    C57BL/6 mouse splenocytes were cultured with ConA/IL-2 for three days. Cells were stained with anti-mouse CD278 (ICOS) (clone 15F9) PE/Fire™ 640 (filled histogram) or left unstained (open histogram).
Compare all formats See PE/Fire™ 640 spectral data
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107713 25 µg 94 CHF
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107714 100 µg 242 CHF
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Description

CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Dong C, et al. 2001. Nature 409(6816):97.
  2. Walker LSK, et al. 2003. J. Immunol. 170:91.
RRID
AB_2924445 (BioLegend Cat. No. 107713)
AB_2924445 (BioLegend Cat. No. 107714)

Antigen Details

Structure
CD28/CTLA-4, 47-57kD
Distribution

Activated T cells, subset of thymocytes

Function
Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor
B7h/B7RP-1/GL-50
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
  2. McAdam AJ, et al. 2000. J. Immunol. 165:5035.
  3. Mak TW, et al. 2003. Nat. Immunol. 4:765
Gene ID
54167 View all products for this Gene ID
UniProt
View information about CD278 on UniProt.org
Go To Top Version: 1    Revision Date: 05.06.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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