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PE Human IgG1 Isotype Ctrl Recombinant Antibody

Pricing & Availability
Clone
QA16A12 (See other available formats)
Regulatory Status
RUO
Other Names
IGHG1, constant region of heavy chain of IgG1, immunoglobulin gamma 1
Isotype
Human IgG1, κ
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Product Citations
publications
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Cat # Size Price Quantity Check Availability Save
403503 25 µg 207 CHF
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403504 100 µg 445 CHF
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Description

Immunoglobulin G1, or IgG1, is a type of antibody. Humans express four types of IgG (IgG1, IgG2, IgG3, and IgG4). Antibodies in mammals are complex proteins, composed of four independent amino acid chains. The molecule is formed by two identical heavy chains and two identical light chains, and produced by B cells. The FcγR molecules (CD16, CD32, and CD64) on the surface of various immune cells can bind IgG1, IgG2, and IgG4.  

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Recombinant
Host Species
Human
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

QA16A12 is a full length recombinant human IgG1 consisting of both heavy and light chains. It can be used as an isotype control when evaluating fully human, humanized, or chimeric antibodies. This product is suitable as an isotype-matched control for ELISA, immunoblotting, FACS, IHC, and functional studies.

Product Citations
  1. Fleischmann M, et al. 2021. Cells. 10:. PubMed
  2. Liu Y, et al. 2022. iScience. 25:104405. PubMed
RRID
AB_3097044 (BioLegend Cat. No. 403503)
AB_3097044 (BioLegend Cat. No. 403504)

Antigen Details

Structure
Immunoglobulin, 150 KDa
Cell Type
B cells
Biology Area
Immunology
Gene ID
NA

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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