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PE/Cyanine7 anti-human C3AR Antibody

Pricing & Availability
Clone
hC3aRZ8 (See other available formats)
Regulatory Status
RUO
Other Names
C3a anaphylatoxin chemotactic receptor, C3a-R, AZ3B, HNFAG09
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
hC3aRZ8_PECyanine7_C3AR_Antibody_1_061719
Human peripheral blood granulocytes were stained with human C3AR (clone hC3aRZ8) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype contrl (open histogram).
  • hC3aRZ8_PECyanine7_C3AR_Antibody_1_061719
    Human peripheral blood granulocytes were stained with human C3AR (clone hC3aRZ8) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype contrl (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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345807 25 tests 183 CHF
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345808 100 tests 382 CHF
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Description

C3AR is a single chain protein of 482 aa, with seven membrane-spanning regions and a molecular weight of 54 Kd. C3AR is expressed by neutrophils, eosinophils, mast cells, monocytes, dendritic cells and there are reports of C3AR expression in non-immune cells such as hematopoietic and mesenchymal stem cells, epithelial and endothelial cells. C3AR is coupled to heterotrimeric G proteins and after C3a binding, the signal transduced, results in chemotaxis, granule enzyme release and superoxide anion production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hC3aR transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Soruri A, et al. 2002. J. Immunol. 170:3306 (FC)
  2. Kiafard Z, et al. 2007 Immunobiology. 212:129 (FC, IHC)
Product Citations
  1. Wohlgemuth L, et al. 2022. Cells. 11: . PubMed
RRID
AB_2810549 (BioLegend Cat. No. 345807)
AB_2810549 (BioLegend Cat. No. 345808)

Antigen Details

Structure
482 aa, single chain protein with seven membrane-spanning regions, 54 kD
Distribution

Neutrophils, eosinophils, mast cells, monocytes, dendritic cells, hematopoietic and mesenchymal stem cells, endothelial cells

Function
After C3a binding, the C3AR signaling results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
G proteins
Bioactivity
Chemotaxis, degranulation
Cell Type
Dendritic cells, Endothelial cells, Eosinophils, Mast cells, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Wysoczynski M et al. 2009. Leukemia. 23:1455
2. Schraufstatter IU. et al. 2009. J. Immunol. 182:3827
3. Honczarenko M et al. 2005. J. Immunol. 175:3698
4. Reca R et al. 2003. Blood 101:3784

Gene ID
719 View all products for this Gene ID
UniProt
View information about C3AR on UniProt.org
Go To Top Version: 2    Revision Date: 06.14.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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