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PE/Cyanine7 anti-human CD197 (CCR7) Antibody

Pricing & Availability
Clone
G043H7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR2, CDw197, EBI1, CMKBR7
Isotype
Mouse IgG2a, κ
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Product Citations
publications
G043H7_PECyanine7_CD197_1_050520
Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
  • G043H7_PECyanine7_CD197_1_050520
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD197 (clone G043H7) PE/Cyanine7 (left) or mouse IgG2a, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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353225 25 tests 183 CHF
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353226 100 tests 382 CHF
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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  3. Edwards CL, et al. 2023. J Clin Invest. 133: . PubMed
  4. Guo A, et al. 2022. Nature. 607:135. PubMed
  5. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  6. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  7. Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed
  8. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
  9. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  10. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  11. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  12. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  13. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  14. Huang X, et al. 2021. Nat Nanotechnol. 16:214. PubMed
  15. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  16. Claireaux M, et al. 2018. MBio. 9:e00317. PubMed
  17. Gao Y, et al. 2021. Oncogenesis. 10:62. PubMed
  18. Schmidt J, et al. 2021. Cell Rep Med. 100194:2. PubMed
  19. Stephenson E, et al. 2021. Nat Med. 27:904. PubMed
  20. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  21. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  22. Mukhopadhyay M, et al. 2017. J Immunol. 10.4049/jimmunol.1700953. PubMed
  23. Swanson E, et al. 2021. eLife. 10:00. PubMed
  24. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  25. Nakasone H, et al. 2021. Commun Biol. 4:1177. PubMed
  26. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  27. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  28. Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed
  29. Chellappa S, et al. 2016. J Leukoc Biol. 100: 5 - 16. PubMed
  30. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  31. Pillet S, et al. 2016. Clin Immunol. 168:72-87. PubMed
  32. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  33. Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
  34. Yang B, et al. 2015. Cell Immunol. Available online 12 August 2015. PubMed
  35. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  36. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
  37. Valle-Casuso JC, et al. 2019. Cell Metab. 29:611. PubMed
  38. Michaels K, et al. 2015. J Immunol. 194:3267. PubMed
  39. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  40. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  41. Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
  42. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  43. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  44. Gannon P, et al. 2015. J Immunol. 195: 356 - 366. PubMed
  45. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  46. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  47. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  48. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  49. Martins AJ, et al. 2017. Cell Systems. 4(4):379-392.e12. PubMed
  50. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
RRID
AB_11126145 (BioLegend Cat. No. 353225)
AB_11126145 (BioLegend Cat. No. 353226)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

View All CD197 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD197 (CCR7) G043H7 FC
Alexa Fluor® 488 anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 421™ anti-human CD197 (CCR7) G043H7 FC
PE anti-human CD197 (CCR7) G043H7 FC
APC/Cyanine7 anti-human CD197 (CCR7) G043H7 FC
Pacific Blue™ anti-human CD197 (CCR7) G043H7 FC
APC anti-human CD197 (CCR7) G043H7 FC
FITC anti-human CD197 (CCR7) G043H7 FC
Alexa Fluor® 647 anti-human CD197 (CCR7) G043H7 FC
PerCP/Cyanine5.5 anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 605™ anti-human CD197 (CCR7) G043H7 FC
PE/Cyanine7 anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 711™ anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 785™ anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 510™ anti-human CD197 (CCR7) G043H7 FC
Brilliant Violet 650™ anti-human CD197 (CCR7) G043H7 FC
PE/Dazzle™ 594 anti-human CD197 (CCR7) G043H7 FC
Biotin anti-human CD197 (CCR7) G043H7 FC
Purified anti-human CD197 (CCR7) (Maxpar® Ready) G043H7 FC,CyTOF®
PerCP anti-human CD197 (CCR7) G043H7 FC
Alexa Fluor® 700 anti-human CD197 (CCR7) G043H7 FC
APC/Fire™ 750 anti-human CD197 (CCR7) G043H7 FC
TotalSeq™-A0148 anti-human CD197 (CCR7) G043H7 PG
TotalSeq™-B0148 anti-human CD197 (CCR7) G043H7 PG
TotalSeq™-C0148 anti-human CD197 (CCR7) G043H7 PG
Brilliant Violet 750™ anti-human CD197 (CCR7) G043H7 FC
Ultra-LEAF™ Purified anti-human CD197 (CCR7) G043H7 FC,Block
Spark NIR™ 685 anti-human CD197 (CCR7) G043H7 FC
KIRAVIA Blue 520™ anti-human CD197 (CCR7) G043H7 FC
PE/Fire™ 640 anti-human CD197 (CCR7) G043H7 FC
Spark YG™ 581 anti-human CD197 (CCR7) G043H7 FC
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody G043H7 FC
TotalSeq™-D0148 anti-human CD197 (CCR7) G043H7 PG
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody G043H7 FC
PE/Cyanine5 anti-human CD197 (CCR7) G043H7 FC
Spark YG™ 593 anti-human CD197 (CCR7) G043H7 FC
PE/Fire™ 744 anti-human CD197 (CCR7) G043H7 FC
Spark Red™ 718 anti-human CD197 (CCR7) (Flexi-Fluor™) G043H7 FC
Spark Red™ 718 anti-human CD197 (CCR7) G043H7 FC
PE anti-human CD197 G043H7 FC
Spark PLUS UV395™ anti-human CD197 (CCR7) G043H7 FC
Go To Top Version: 2    Revision Date: 04.08.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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