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PE/Cyanine7 anti-human GPR183 (EBI2) Antibody

Pricing & Availability
Clone
SA313E4 (See other available formats)
Regulatory Status
RUO
Other Names
EBI2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
SA313E4_PECyanine7_GPR183_Antibody_062320
Human peripheral blood lymphocytes were stained with CD19 Pacific Blue™ and GPR183 (EBI2) (clone SA313E4) PE/Cyanine7 (left) or mouse IgG2a, κ isotype control PE/Cyanine7 (right).
  • SA313E4_PECyanine7_GPR183_Antibody_062320
    Human peripheral blood lymphocytes were stained with CD19 Pacific Blue™ and GPR183 (EBI2) (clone SA313E4) PE/Cyanine7 (left) or mouse IgG2a, κ isotype control PE/Cyanine7 (right).
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368915 25 tests 188 CHF
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368916 100 tests 405 CHF
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Description

GPR183, also known as EBI2, is a member of the rhodopsin-like subfamily of 7TM receptors, which forms homodimers and heterodimers when it associates with CXCR5. GPR183 is expressed by Naive B cells, subset of T cells, monocytes and macrophages, and is highly upregulated by Epstein–Barr virus infection. GPR183 regulates the B cell trafficking within lymphoid follicles in response to 7α, 25-dihydroxycholesterol.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human GPR183-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876697 (BioLegend Cat. No. 368915)
AB_2876697 (BioLegend Cat. No. 368916)

Antigen Details

Structure
Member of the rhodopsin-like subfamily of 7TM receptors and forms homo- and heterodimers.
Distribution

Naive B cells, subset of T cells, monocytes and macrophages

Function
Chemotactic receptor that regulates B cell trafficking within lymphoid follicles.
Ligand/Receptor
7α, 25-dihydroxycholesterol
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokine/Chemokine Receptors, Steroid Receptors/Nuclear Receptors
Antigen References

1. Preuss I, et al. 2014. Biochem. Biophys. Res. Commun. 446:663.
2. Barroso R, et al. 2012. FASEB J. 26:4841.
3. Hannedouche S, et al. 2011. Nature. 475:524.
4. Benned-Jensen T, et al. 2011. J. Biol. Chem. 286:29292.

Gene ID
1880 View all products for this Gene ID
UniProt
View information about GPR183 on UniProt.org
Go To Top Version: 1    Revision Date: 06.23.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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