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PerCP anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RM4-5_PerCP_011508
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PerCP (filled histogram) or rat IgG2a, κ PerCP isotype control (open histogram).
  • RM4-5_PerCP_011508
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PerCP (filled histogram) or rat IgG2a, κ PerCP isotype control (open histogram).
Compare all formats See PerCP spectral data
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100537 25 µg 99 CHF
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100538 100 µg 237 CHF
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  2. Cha JH, et al. 2022. J Biol Chem. 298:101817. PubMed
  3. Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed
  4. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  5. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  6. Yang R, et al. 2020. Cell Death Dis. 0.877083333. PubMed
  7. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  8. Martens R, et al. 2020. Nat Commun. 11:1114. PubMed
  9. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  10. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  11. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  12. Tian H, et al. 2022. Cancer Sci. 113:875. PubMed
  13. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  14. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  15. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  16. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  17. Roy S, et al. 2020. Sci Rep. 10:10992. PubMed
  18. Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed
  19. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  20. Thauland T, et al. 2014. J Immunol. 193:5894. PubMed
  21. Köchl R, et al. 2020. Elife. 9:00. PubMed
  22. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  23. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  24. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  25. Jacque E, et al. 2015. J Exp Med. 212:883. PubMed
  26. Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
  27. Sood S, et al. 2016. J Immunol. 197: 429 - 440. PubMed
  28. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  29. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  30. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  31. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  32. Riva A, et al. 2017. PLoS One.. 10.1371/journal.pone.0181964. PubMed
  33. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  34. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  35. Hartwig S, et al. 2014. PLoS One. 9:90720. PubMed
  36. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  37. Menon M, et al. 2014. PLoS Genet. 10:1004558. PubMed
  38. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  39. Tao Z, et al. 2022. Cells. 11:. PubMed
  40. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  41. Jacque E, et al. 2014. J Exp Med. 211:2085. PubMed
  42. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
  43. Sturmlechner I, et al. 2021. Science. 374:eabb3420. PubMed
  44. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  45. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  46. Yuan M, et al. 2017. Hum Reprod. 32:94. PubMed
  47. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID
AB_893325 (BioLegend Cat. No. 100537)
AB_893325 (BioLegend Cat. No. 100538)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 3    Revision Date: 01.29.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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