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PerCP/Cyanine5.5 anti-Cleaved PARP (Asp214) Recombinant Antibody

Pricing & Availability
Clone
QA17A17 (See other available formats)
Regulatory Status
RUO
Other Names
PARP1, Poly (ADP-ribose) polymerase, PPOL, ARTD1, ADPRT, EC 2.4.2, ADP-Ribosyltransferase (NAD+;Poly (ADP-Ribose) Polymerase), Poly (ADP-Ribose) Polymerase Family, Member 1, DNA ADP-Ribosyltransferase PARP1, Poly[ADP-Ribose] Synthase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A17_-PERCP_CYANINE55_-Cleaved-PARP_Antibody_1_012421
Human T leukemia cell line Jurkat treated (filled histogram) or non-treated (open histogram) with Ultra-LEAF™ purified anti-human CD95 (Cat. No. 305705) for 4 hours, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-Cleaved PARP (Asp214) recombinant (clone QA17A17) PerCP/Cyanine5.5.
  • QA17A17_-PERCP_CYANINE55_-Cleaved-PARP_Antibody_1_012421
    Human T leukemia cell line Jurkat treated (filled histogram) or non-treated (open histogram) with Ultra-LEAF™ purified anti-human CD95 (Cat. No. 305705) for 4 hours, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-Cleaved PARP (Asp214) recombinant (clone QA17A17) PerCP/Cyanine5.5.
  • QA17A17_-PERCP_CYANINE55_-Cleaved-PARP_Antibody_3_012421
    Human T leukemia cell line Jurkat treated (right) or non-treated (left) with Ultra-LEAF™ purified anti-human CD95 (Cat. No. 305705) for 4 hours, stained with Zombie Violet™ (Cat. No. 423114) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-Cleaved PARP (Asp214) recombinant (clone QA17A17) PerCP/Cyanine5.5.
Compare all formats See PerCP/Cyanine5.5 spectral data
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669905 25 tests 188 CHF
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669906 100 tests 422 CHF
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Description

PARP (ADP-Ribose) Polymerase (PARP) is a 113 kD nuclear protein important for mediating the normal cellular response to DNA damage and repair in response to environmental stress. PARP catalyzes the transfer of ADP-ribose units from NAD⁺ to various nuclear proteins, including topoisomerases, histones, and PARP itself, by poly(ADP-ribosyl)ation. Following DNA damage, this protein is cleaved by ICE-like caspases, such as caspase-3 and -7. In humans, this PARP cleavage occurs between Asp214 and Gly215, yielding an 89 kD (from the carboxy-terminal catalytic domain) and a 24 kD fragment (from the amino-terminal DNA binding domain). PARP is an important regulator of cellular differentiation, proliferation, and tumor transformation, and PARP cleavage is considered a classical characteristic of cells undergoing apoptosis. Additionally, this enzyme may be the site of mutation in Fanconi anemia, and may contribute to the pathophysiology of type I diabetes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Proprietary synthetic peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC – Quality tested 
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Application Notes

The QA17A17 antibody reacts with the 89 kD (cleaved at Asp214) fragment of human PARP1. It does not react with full-length PARP1.

Fixation and permeabilization using the True-Nuclear Buffer Set and Intracellular Staining Permeabilization Wash Buffer (10X) have also been confirmed to work in addition to the True-Phos™ Perm Buffer during in-house testing and development to detect cleaved PARP (Asp214).

RRID
AB_2910493 (BioLegend Cat. No. 669905)
AB_2910493 (BioLegend Cat. No. 669906)

Antigen Details

Structure
The large fragment of cleaved PARP is an 801 amino acid product with a predicted molecular weight of 89 kD
Distribution

Nuclear

Function
Molecular “nick sensor”; base excision repair; catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture; DNA metabolism; protein modification may enhance or repress transcription
Interaction
Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modified TATA-BP, YY1, Sp1, NF-B, p53 and others
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Satoh M, Lindahl T. 1992. Nature. 356:356. PubMed
  2. Kaufmann S, et al. 1993. Cancer Res. 53:3976. PubMed
  3. Lazebnik Y, et al. 1994. Nature. 371:347. PubMed
  4. D’Amours D, et al. 1999. Biochem J. 342:249. PubMed
  5. Chaitanya G, et al. 2010. Cell Commun Signal. 8:31. PubMed
Gene ID
142 View all products for this Gene ID
UniProt
View information about Cleaved PARP on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 01.24.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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