- Clone
- W19195B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![UCHT1_APC_GATA3_PerCP-Cyanine55-His_122823 UCHT1_APC_GATA3_PerCP-Cyanine55-His_122823](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/UCHT1_APC_GATA3_PerCP-Cyanine55-His_122823-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Murine Th2 cell line D10.G4.1 was fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) PerCP/Cyanine5.5 (filled histogram), or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram). -
Th2 polarized human peripheral blood lymphocytes were surface stained with anti-human CD3 (clone UCHT1) APC/Fire™ 750. Cells were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401), and intracellularly stained with anti-GATA3 (clone W19195B) PerCP/Cyanine5.5 or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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386909 | 25 tests | 155 CHF | ||||
386910 | 100 tests | 390 CHF |
GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines. This is responsible for the preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4+ T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defects in GATA3 result in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial human GATA3 recombinant protein (1-258 aa)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Clone W19195B does not block 16E10A23, TWAJ, or L50-823.
- RRID
-
AB_3097430 (BioLegend Cat. No. 386909)
AB_3097430 (BioLegend Cat. No. 386910)
Antigen Details
- Structure
- 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus
- Distribution
-
Nucleus
- Function
- GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T) GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation
- Interaction
- Interacts with Smad3, FOG1, FOG2, and PU.1
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Yagi R, et al. 2011. Int Immunol. 23:415-20.
- Chou J, et al. 2010. J Cell Physiol. 222:42-9.
- Ho IC, et al. 2009. Nat Rev Immunol. 9:125-35.
- Rothenberg EV, et al. 2008. Semin Immunol. 20:236-46.
- Kouros-Mehr H, et al. 2008. Curr Opin Cell Biol. 20:164-70.
- Van Esch H, et al. 2000. Nature. 406:419-22.
- Gene ID
- 2625 View all products for this Gene ID
- UniProt
- View information about GATA3 on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All GATA3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GATA3 | W19195B | ICFC |
PE anti-GATA3 | W19195B | ICFC |
APC/Fire™ 750 anti-GATA3 | W19195B | ICFC |
APC anti-GATA3 | W19195B | ICFC |
PerCP/Cyanine5.5 anti-GATA3 | W19195B | ICFC |
Brilliant Violet 421™ anti-GATA3 | W19195B | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-GATA3
Th2 polarized human peripheral blood lymphocytes were treate... Murine Th2 cell line D10.G4.1 was treated with True-Nuclear™... -
PE anti-GATA3
Th2 polarized Human peripheral blood lymphocytes were surfac... Murine Th2 cell line D10.G4.1 was fixed and permeabilized wi... -
APC/Fire™ 750 anti-GATA3
Murine Th2 cell line D10.G4.1 was fixed and permeabilized wi... Th2 polarized human peripheral blood lymphocytes were surfac... -
APC anti-GATA3
Murine Th2 cell line D10.G4.1 was fixed and permeabilized wi... Th2 polarized human peripheral blood lymphocytes were surfac... -
PerCP/Cyanine5.5 anti-GATA3
Murine Th2 cell line D10.G4.1 was fixed and permeabilized wi... Th2 polarized human peripheral blood lymphocytes were surfac... -
Brilliant Violet 421™ anti-GATA3
Murine Th2 cell line D10.G4.1 was fixed and permeabilized wi... Th2 polarized human peripheral blood lymphocytes were surfac...
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