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PerCP/Cyanine5.5 anti-human CD34 Antibody

Pricing & Availability
Clone
561 (See other available formats)
Regulatory Status
RUO
Other Names
Gp105-120, My10, Mucosialin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
561_PerCPCyanine55_CD34_Antibody_110218
Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 APC, and CD34 (clone 561) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Dot plots are gated on CD14- cells.
  • 561_PerCPCyanine55_CD34_Antibody_110218
    Human peripheral blood mononuclear cells were stained with CD14 PE, CD45 APC, and CD34 (clone 561) PerCP/Cyanine5.5 (left), or mouse IgG2a, κ PerCP/Cyanine5.5 isotype control (right). Dot plots are gated on CD14- cells.
Compare all formats See PerCP/Cyanine5.5 spectral data
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343611 25 tests 171 CHF
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343612 100 tests 382 CHF
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed
  2. Lina IA, et al. 2022. Otolaryngol Head Neck Surg. 166:712. PubMed
  3. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  4. Bao EL, et al. 2020. Nature. 769:586. PubMed
  5. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  6. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  7. Kiehlmeier S, et al. 2021. Leukemia. Online ahead of prin. PubMed
  8. Rodriguez‐Meira A et al. 2019. Molecular cell. 73(6):1292-1305 . PubMed
RRID
AB_2566787 (BioLegend Cat. No. 343611)
AB_2566787 (BioLegend Cat. No. 343612)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11.02.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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