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PerCP/Cyanine5.5 anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_PerCPCyanine55_CD4_Antibody_120518
Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • RPA-T4_PerCPCyanine55_CD4_Antibody_120518
    Human peripheral blood lymphocytes were stained with CD4 (clone RPA-T4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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300529 25 tests 112 CHF
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300530 100 tests 218 CHF
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  2. Qiu C, et al. 2022. Front Bioeng Biotechnol. 10:1027619. PubMed
  3. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  4. Gallucci L, et al. 2023. EMBO Rep. 24:e56818. PubMed
  5. Wu X, et al. 2023. iScience. 26:106559. PubMed
  6. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
  7. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  8. Chan JA, et al. 2022. Nat Commun. 13:4159. PubMed
  9. Zheng J, et al. 2021. J Infect Dis. 223:785. PubMed
  10. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  11. Emert-Sedlak LA, et al. 2022. ACS Infect Dis. 8:91. PubMed
  12. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  13. Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed
  14. Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed
  15. Lu T, et al. 2016. PLoS One. 11: 0148044. PubMed
  16. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  17. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  18. Du L, et al. 2021. Front Mol Biosci. 8:675179. PubMed
  19. Woldemeskel BA, et al. 2021. J Clin Invest. 131:. PubMed
  20. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  21. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  22. Woldemeskel BA, et al. 2022. J Clin Invest. 132:. PubMed
  23. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  24. Robinson CA, et al. 2022. Viruses. 14:. PubMed
  25. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  26. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  27. Zakhour R, et al. 2016. Clin Infect Dis. 62: 1029-1035. PubMed
  28. Elias G, et al. 2022. Elife. 11:. PubMed
  29. Sumitomo S, et al. 2013. J Immunol. 94:393. PubMed
  30. Pollack RA, et al. 2017. Cell Host Microbe. 1.218055556. PubMed
  31. Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
  32. Dykema AG, et al. 2021. J Clin Invest. 131:. PubMed
  33. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  34. D’Antoni ML, et al. 2018. J Acquir Immune Defic Syndr. 79:108. PubMed
  35. Wiche Salinas TR, et al. 2021. iScience. 24:103225. PubMed
  36. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  37. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  38. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  39. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID
AB_893322 (BioLegend Cat. No. 300529)
AB_893322 (BioLegend Cat. No. 300530)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD4 RPA-T4 FC
Biotin anti-human CD4 RPA-T4 FC
FITC anti-human CD4 RPA-T4 FC,SB
PE anti-human CD4 RPA-T4 FC
PE/Cyanine5 anti-human CD4 RPA-T4 FC
PE/Cyanine7 anti-human CD4 RPA-T4 FC
Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
APC/Cyanine7 anti-human CD4 RPA-T4 FC
Alexa Fluor® 488 anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 647 anti-human CD4 RPA-T4 FC,SB
Pacific Blue™ anti-human CD4 RPA-T4 FC
Brilliant Violet 421™ anti-human CD4 RPA-T4 FC,ICC
Alexa Fluor® 700 anti-human CD4 RPA-T4 FC,SB
PerCP anti-human CD4 RPA-T4 FC
PerCP/Cyanine5.5 anti-human CD4 RPA-T4 FC
Brilliant Violet 570™ anti-human CD4 RPA-T4 FC
Brilliant Violet 650™ anti-human CD4 RPA-T4 FC
Purified anti-human CD4 (Maxpar® Ready) RPA-T4 FC,CyTOF®
Alexa Fluor® 594 anti-human CD4 RPA-T4 FC,ICC
Brilliant Violet 510™ anti-human CD4 RPA-T4 FC
PE/Dazzle™ 594 anti-human CD4 RPA-T4 FC
Brilliant Violet 785™ anti-human CD4 RPA-T4 FC
Brilliant Violet 605™ anti-human CD4 RPA-T4 FC
Brilliant Violet 711™ anti-human CD4 RPA-T4 FC
APC/Fire™ 750 anti-human CD4 RPA-T4 FC
CD4 Fluorophore Sampler Kit RPA-T4 FC
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC RPA-T4 FC
TotalSeq™-A0072 anti-human CD4 RPA-T4 PG
TotalSeq™-B0072 anti-human CD4 RPA-T4 PG
TotalSeq™-C0072 anti-human CD4 RPA-T4 PG
Ultra-LEAF™ Purified anti-human CD4 RPA-T4 FC,CyTOF®,Activ,Block,IHC
TotalSeq™-D0072 anti-human CD4 RPA-T4 PG
Go To Top Version: 3    Revision Date: 12.05.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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