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PerCP/Cyanine5.5 anti-human CD80 Antibody

Pricing & Availability
Clone
2D10 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD80.1
Other Names
B7-1, B7, BB1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
2D10_PerCPCy5.5_CD80_Antibody_FC_101915
Human B-cell Burkitt's lymphoma cell line Raji was stained with CD80 (clone 2D10) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 2D10_PerCPCy5.5_CD80_Antibody_FC_101915
    Human B-cell Burkitt's lymphoma cell line Raji was stained with CD80 (clone 2D10) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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305231 25 tests 118 CHF
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305232 100 tests 262 CHF
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Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Lei Y, et al. 2024. Nat Commun. 113:15. PubMed
  2. Hsu SPC, et al. 2020. J Neurooncol. 146:417. PubMed
  3. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  4. Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed
  5. Yu X, et al. 2020. Cancer Cell. 37(6):850-866. PubMed
  6. Flieswasser T, et al. 2020. Cells. 9:00. PubMed
  7. Shrestha P, et al. 2021. PLoS Pathog. 17:e1009091. PubMed
  8. Krakow S, et al. 2019. Front Immunol. 10:1484. PubMed
  9. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  10. Riegel K, et al. 2021. Cell Death Dis. 12:969. PubMed
  11. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
RRID
AB_2566490 (BioLegend Cat. No. 305231)
AB_2566490 (BioLegend Cat. No. 305232)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution

Activated B cells and T cells, macrophages, and dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 10.19.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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