- Clone
- 3D12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HLA class I histocompatibility antigen, alpha chain E, HLAE
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3D12_PerCPCy5.5_HLA-E_Antibody_FC_012315 3D12_PerCPCy5.5_HLA-E_Antibody_FC_012315](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3D12_PerCPCy5.5_HLA-E_Antibody_FC_012315.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with HLA-E (clone 3D12) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
342609 | 25 tests | 159 CHF | ||||
342610 | 100 tests | 345 CHF |
HLA-E is a non-classical MHC class I (MHC-Ib) molecule. It is characterized by limited polymorphism and ubiquitous expression. HLA-E, as other MHC-I molecules, is heterodimerized with β2-microglobulin. HLA-E interacts with CD94/NKG2 receptor to regulate NK cell cytolysis activities.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
-
Application References
(PubMed link indicates BioLegend citation) -
- Lee N, et al. 1998. Proc. Natl. Acad. Sci. USA. 95:5199.
- Wooden SL, et al. 2005. J. Immunol. 175:1383.
- Monaco EL, et al. 2008. J. Immunol. 181:5442.
- Corrah TW, et al. 2011. J. Virol. 85:3367. PubMed
- Product Citations
-
- RRID
-
AB_2565434 (BioLegend Cat. No. 342609)
AB_2565435 (BioLegend Cat. No. 342610)
Antigen Details
- Structure
- HLA family, Ig superfamily, 42 kD
- Distribution
-
Broadly expressed
- Function
- Mediate cell recognition by NK cells
- Ligand/Receptor
- CD94/NKG2
- Biology Area
- Immunology
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Sullivan LC, et al. 2008. Tissue Antigen. 72:415.
2. Koller BH, et al. 1988. J. Immunol. 141:897. - Gene ID
- 3133 View all products for this Gene ID
- UniProt
- View information about HLA-E on UniProt.org
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
-
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
View All HLA-E Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human HLA-E | 3D12 | FC,IP |
PE anti-human HLA-E | 3D12 | FC |
APC anti-human HLA-E | 3D12 | FC |
PE/Cyanine7 anti-human HLA-E | 3D12 | FC |
PerCP/Cyanine5.5 anti-human HLA-E | 3D12 | FC |
Brilliant Violet 421™ anti-human HLA-E | 3D12 | FC |
PE/Dazzle™ 594 anti-human HLA-E | 3D12 | FC |
TotalSeq™-A0918 anti-human HLA-E | 3D12 | PG |
TotalSeq™-C0918 anti-human HLA-E | 3D12 | PG |
Biotin anti-human HLA-E | 3D12 | FC |
TotalSeq™-B0918 anti-human HLA-E | 3D12 | PG |
TotalSeq™-D0918 anti-human HLA-E | 3D12 | PG |
Brilliant Violet 605™ anti-human HLA-E | 3D12 | FC |
Brilliant Violet 650™ anti-human HLA-E | 3D12 | FC |
Brilliant Violet 711™ anti-human HLA-E | 3D12 | FC |
Brilliant Violet 785™ anti-human HLA-E | 3D12 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human HLA-E
Human peripheral blood lymphocytes stained with 3D12 PE -
PE anti-human HLA-E
Human peripheral blood lymphocytes stained with 3D12 PE -
APC anti-human HLA-E
Human peripheral blood lymphocytes were stained with HLA-E (... -
PE/Cyanine7 anti-human HLA-E
Human peripheral blood lymphocytes were stained with HLA-E (... -
PerCP/Cyanine5.5 anti-human HLA-E
Human peripheral blood lymphocytes were stained with HLA-E (... -
Brilliant Violet 421™ anti-human HLA-E
Human peripheral blood lymphocytes were stained with HLA-E (... -
PE/Dazzle™ 594 anti-human HLA-E
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-A0918 anti-human HLA-E
-
TotalSeq™-C0918 anti-human HLA-E
-
Biotin anti-human HLA-E
Human peripheral blood lymphocytes were stained with biotin... -
TotalSeq™-B0918 anti-human HLA-E
-
TotalSeq™-D0918 anti-human HLA-E
-
Brilliant Violet 605™ anti-human HLA-E
Human lysed whole blood was stained with anti-human HLA-E (c... Human lysed whole blood was stained with anti-human HLA-E (c... -
Brilliant Violet 650™ anti-human HLA-E
Human lysed whole blood was stained with anti-human HLA-E (c... -
Brilliant Violet 711™ anti-human HLA-E
Human peripheral blood lymphocytes were stained with anti-hu... Human peripheral blood lymphocytes, monocytes & granulocytes... -
Brilliant Violet 785™ anti-human HLA-E
Human peripheral blood lymphocytes were stained with anti-hu... Human peripheral blood lymphocytes were stained with anti-hu...
Follow Us