PerCP/Cyanine5.5 anti-human S100A4 Antibody anti-S100A4

Pricing & Availability

Clone: NJ-4F3-D1 (See other available formats)
Regulatory Status: RUO
Other Names: S100 Calcium Binding Protein A4, 42A, 18A2, CAPL, FSP1, MTS1, P9KA, PEL98, Calvasculin, Metastasin, Placental calcium-binding protein
Isotype: Mouse IgG1, κ
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NJ-4F3-D1_PerCPCyanine55_S100A4_Antibody_013019 — Human peripheral blood mononuclear cells were stimulated with PMA+ionomycin for 10 minutes, then fixed, permeabilized and stained with S100A4 (clone NJ-4F3-D1) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (op

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370009

25 tests

176 CHF

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370010

100 tests

405 CHF

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Description

S100A4 is a member of the S100 family of calcium-binding proteins that contain two Ca2⁺-binding EF-hand motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It can be secreted by unknown mechanisms, stimulating a variety of cellular responses, including angiogenesis and neuronal growth. S100A4 has been shown to be a prognostic marker in a number of human cancers. It also has a potential use as a marker for tumor metastasis and disease progression.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: ELISA Detection.
RRID: AB_2572075 (BioLegend Cat. No. 370009)
AB_2572075 (BioLegend Cat. No. 370010)

Antigen Details

Structure: EF-hand motif.
Distribution: Widely expressed; localized in the cytoplasm and/or nucleus of a vast range of cells.
Function: Calcium-binding.
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: Immune Checkpoint Receptors
Antigen References: 1. Schneider M, et al. 2008. J. Mol. Med. 86:507.
2. Donato R, et al. 2013. Curr. Mol. Med. 13:24.
3. Sherbet GV. 2009. Cancer Lett. 280:15.
Gene ID: 6275 View all products for this Gene ID
UniProt: View information about S100A4 on UniProt.org

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Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All S100A4 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human S100A4	NJ-4F3-D1	ICFC
Purified anti-human S100A4	NJ-4F3-D1	ICFC,ELISA Detection
FITC anti-human S100A4	NJ-4F3-D1	ICFC
PerCP/Cyanine5.5 anti-human S100A4	NJ-4F3-D1	ICFC
APC anti-human S100A4	NJ-4F3-D1	ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 01.30.2019

For Research Use Only. Not for diagnostic or therapeutic use.

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