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PerCP/Cyanine5.5 anti-mouse CD204 Antibody

Pricing & Availability
Clone
1F8C33 (See other available formats)
Regulatory Status
RUO
Other Names
Msr1, MSR, SR-A, Scavenger receptor type A, Macrophage scavenger receptor I, Scara1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1F8C33_PerCPCy5dot5_CD204_Antibody_042321.png
Mouse leukemic monocyte-macrophage cell line RAW267.4 was stained with PerCP/Cyanine5.5 anti-mouse CD204 (clone 1F8C33) (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 1F8C33_PerCPCy5dot5_CD204_Antibody_042321.png
    Mouse leukemic monocyte-macrophage cell line RAW267.4 was stained with PerCP/Cyanine5.5 anti-mouse CD204 (clone 1F8C33) (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
154715 25 µg 194 CHF
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154716 100 µg 434 CHF
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Description

CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is 220 kDa, trimeric type II transmembrane protein, with one scavenger receptor cysteine-rich domain (SRCR). It is a phagocytic pattern-recognition receptor (PRR) expressed on macrophages and dendritic cells. CD204 is a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and plays a critical role in atherogenesis. CD204 also recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD204 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

This clone has minimal recognition of CD204 in C57BL/6.

RRID
AB_2892313 (BioLegend Cat. No. 154715)
AB_2892313 (BioLegend Cat. No. 154716)

Antigen Details

Structure
Trimeric type II transmembrane protein, one scavenger receptor cysteine-rich domain (SRCR), 220 kD.
Distribution

Macrophages, dendritic cells.

Function
Uptake of negatively charged molecules, role in pathology of atherosclerosis and Alzheimer’s disease.
Ligand/Receptor
Acetylated and oxidized lipoproteins
Cell Type
Dendritic cells, Macrophages
Antigen References
  1. Shichita T, et al. 2017. Nat Med. 23:723.
  2. Muczynski V, et al. 2017. Blood. 129:2443.
  3. Iftakhar-E-Khuda I, et al. 2016. Proc Natl Acad Sci U S A. 113:10643.
  4. Nellimarla S, et al. 2015. J Immunol. 195:3858.
  5. Bonilla DL, et al. 2013. Immunity. 39:537.
Gene ID
20288 View all products for this Gene ID
UniProt
View information about CD204 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06.15.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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