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PerCP/Cyanine5.5 anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody

Pricing & Availability
Clone
m2B4 (B6)458.1 (See other available formats)
Regulatory Status
RUO
Other Names
2B4 B6 Alloantigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
m2B4_PerCPCyanine55_CD244-2_Antibody_1_112123
Human peripheral blood lymphocytes were stained with CD49b FITC and CD244.2 (clone m2B4 (B6)458.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • m2B4_PerCPCyanine55_CD244-2_Antibody_1_112123
    Human peripheral blood lymphocytes were stained with CD49b FITC and CD244.2 (clone m2B4 (B6)458.1) PerCP/Cyanine5.5 (left) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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133513 25 µg 176 CHF
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133514 100 µg 382 CHF
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Description

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
m2B4-Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Lee KM, et al. 2006. Blood. 107:3181.
  2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
  3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
  4. de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed
RRID
AB_2564341 (BioLegend Cat. No. 133513)
AB_2564341 (BioLegend Cat. No. 133514)

Antigen Details

Structure
A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution

All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.

Function
Mediate non-MHC-restricted cytotoxicity.
Ligand/Receptor
CD48
Cell Type
Dendritic cells, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
18106 View all products for this Gene ID
UniProt
View information about CD244.2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11.03.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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