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PerCP/Cyanine5.5 anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
390_PerCPCyanine55_CD31_Antibody_111918
C57BL/6 mouse splenocytes were stained with CD31 (clone 390) PerCP/Cyanine5.5 (open histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • 390_PerCPCyanine55_CD31_Antibody_111918
    C57BL/6 mouse splenocytes were stained with CD31 (clone 390) PerCP/Cyanine5.5 (open histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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102419 25 µg 124 CHF
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102420 100 µg 375 CHF
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  2. Wang L, et al. 2022. Cell Metab. 34:75. PubMed
  3. Zhang Q, et al. 2022. Nat Metab. 4:1055. PubMed
  4. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  5. Sun C, et al. 2020. Cell Stem Cell. 26(5):707-721. PubMed
  6. Lin J, et al. 2021. Front Immunol. 12:796260. PubMed
  7. Shen H, et al. 2022. Nat Commun. 13:6030. PubMed
  8. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  9. Angelidis I, et al. 2019. Nat Commun. 10:963. PubMed
  10. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  11. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  12. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  13. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  14. Li X, et al. 2022. Bone Res. 10:46. PubMed
  15. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  16. Parker KR, et al. 2020. Cell. 183(1):126-142.e17. PubMed
  17. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  18. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  19. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  20. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  21. van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed
  22. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  23. Shan B, et al. 2020. Nat Metab. 1332:2. PubMed
  24. Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed
  25. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  26. Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed
  27. Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed
  28. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  29. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  30. Tal R, et al. 2019. Biol Reprod. 100:1228. PubMed
  31. Wang Y, et al. 2020. Theranostics. 6.736111111. PubMed
  32. Prados A, Kollias G, Koliaraki V 2016. Sci Rep. 6: 33027. PubMed
  33. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  34. Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  35. Noll J, et al. 2014. Haematologica. 99:163. PubMed
RRID
AB_10613644 (BioLegend Cat. No. 102419)
AB_10613644 (BioLegend Cat. No. 102420)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 11.19.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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