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PerCP/Cyanine5.5 anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_PerCPCy5.5_052610
Thioglycolate-elicited BALB/c macrophages were fixed, permeabilized, and intracellularly stained with CD68 (clone FA-11) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • FA-11_PerCPCy5.5_052610
    Thioglycolate-elicited BALB/c macrophages were fixed, permeabilized, and intracellularly stained with CD68 (clone FA-11) PerCP/Cyanine5.5 (filled histogram) or rat IgG2a, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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137009 25 µg 159 CHF
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137010 100 µg 370 CHF
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed
  2. Li Y, et al. 2022. Brain Behav Immun. 101:1. PubMed
  3. Liang P, et al. 2022. Prostate Cancer Prostatic Dis. 25:539. PubMed
  4. Gruber R, et al. 2014. J Neurosci. 34:16320. PubMed
  5. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  6. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  7. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  8. Kokona D, et al. 2016. Exp Eye Res. S0014-4835(16)30499-7. PubMed
  9. Oliver J, et al. 2012. Am J Physiol Renal Physiol. 302:F1362. PubMed
  10. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
  11. Lu A, et al. 2020. Sci Rep. 10:7075. PubMed
  12. Simonneau M, et al. 2018. Oncotarget. 9:36457. PubMed
  13. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  14. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  15. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  16. Miura I, et al. 2021. Physiol Rep. 9:e14859. PubMed
  17. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  18. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  19. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  20. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  21. Komine S, et al. 2017. Sci Rep. 10.1038/s41598-017-12358-8. PubMed
  22. Nasti A, et al. 2017. Eur J Immunol. 47:2163. PubMed
RRID
AB_2260046 (BioLegend Cat. No. 137009)
AB_2260046 (BioLegend Cat. No. 137010)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01.03.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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