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PerCP/Cyanine5.5 anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
H1dot2F3_PerCPCyanine55_CD69_Antibody_091919.png
PMA+ionomycin stimulated (6 hours) C57BL/6 mouse splenocytes stained with CD69 (clone H1.2F3) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram)
  • H1dot2F3_PerCPCyanine55_CD69_Antibody_091919.png
    PMA+ionomycin stimulated (6 hours) C57BL/6 mouse splenocytes stained with CD69 (clone H1.2F3) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram)
Compare all formats See PerCP/Cyanine5.5 spectral data
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104521 25 µg 118 CHF
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104522 100 µg 309 CHF
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  2. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  3. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  4. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  5. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  6. Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
  7. Nijnik A, et al. 2009. J Immunol. 183:5788. PubMed
  8. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  9. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  10. Amor C, et al. 2020. Nature. 583:127. PubMed
  11. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  12. Schofield DJ, et al. 2021. MAbs. 13:1857100. PubMed
  13. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  14. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  15. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  16. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  17. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  18. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  19. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  20. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  21. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  22. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  23. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  24. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  25. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  26. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  27. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  28. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  29. Tomaru U et al. 2019. Cell reports. 26(3):639-651 . PubMed
  30. Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
RRID
AB_2260065 (BioLegend Cat. No. 104521)
AB_2260065 (BioLegend Cat. No. 104522)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 09.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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